Influence of green light illumination on several enzymes involved in energy metabolism in the oriental armyworm, Mythimna separata (Lepidoptera: Noctuidae)
Influence of green light illumination on several enzymes involved in energy metabolism in the oriental armyworm, Mythimna separata (Lepidoptera: Noctuidae)
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绿光照明对东方粘虫 Mythimna separata（鳞翅目：夜蛾科）能量代谢中涉及的几种酶的影响
DOI：
10.1016/j.aspen.2019.03.002
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发表时间：
2019-06-01
影响因子：
1.5
通讯作者：
Lei, Chao-Liang
中科院分区：
文献类型：
--
作者：
Kim, Kil-Nam;Sin, Un-Chol;Lei, Chao-Liang
东方粘虫Mythimna separata步行者是东亚地区的重要害虫。绿色（520 nm）光在> 30 min的曝光时间下可导致比其它波长的光更强的趋光反应。分离蛾本研究主要是测定M.的几种参与能量代谢的酶（如甘油醛-3-磷酸脱氢酶（GAPDH）、甘油-3-磷酸脱氢酶（GPDH）、乳酸脱氢酶（LDH）和3-羟酰辅酶A脱氢酶（HOAD））的活性以及HOAD与GAPDH活性的比值。用不同曝光时间的绿色光照射粘虫蛾。结果表明，当M.绿色光照射30 min后，小菜蛾体内GPDH和HOAD两种酶的活性以及HOAD/GAPDH的比值均显著升高。60 min处理组的LDH活性显著升高，GAPDH活性在45 min处理组、LDH活性在各处理组、HOAD/GAPDH活性在45 min处理组雌雄蛾间差异显著。我们建议当M.将粘虫成虫暴露于绿色光，这些酶可被激活以产生用于启动对绿色光的趋光行为的能量。本研究结果为阐明夜行蛾趋光行为的原因提供了理论依据。
The oriental armyworm, Mythimna separata Walker, is an important insect pest in eastern Asia. Green (520 nm) light with an exposure time of > 30 min can result in a stronger phototactic response than the wavelengths of other lights in M. separata moths. The present study was mainly focused to estimate the activities of several enzymes (such as glyceraldehyde-3-phosphate dehydrogenase (GAPDH), glycerol-3-phosphate dehydrogenase (GPDH), lactate dehydrogenase (LDH) and 3-hydroxyacyl-CoA dehydrogenase (HOAD)) involved in the energy metabolism and a ratio of HOAD to GAPDH activity in M. separata moths exposed to the green light for different exposure times. Our results showed that when M. separata moths were exposed to the green light for 30 min, the activities of the two enzymes (GPDH and HOAD) and as well as the ratio of HOAD to GAPDH activity were significantly elevated. The activity of LDH was significantly increased in the moths exposed for 60 min. Furthermore, significant differences in enzyme activities between the male and female moths were recorded in 45 min exposure time group of the GAPDH, all light treatment groups of the LDH, and 45 min group of the ratio of HOAD to GAPDH activity, respectively. We suggest that when M. separata adult moths are exposed to the green light, these enzymes can be activated to produce energy for starting the phototactic behavior to the green light. Our findings may provide a theoretical basis for elucidating a reason of the phototactic behavior of nocturnal moths.