GLYCOGEN DEPLETION PATTERN IN HUMAN SKELETAL-MUSCLE FIBERS AFTER HEAVY EXERCISE
GLYCOGEN DEPLETION PATTERN IN HUMAN SKELETAL-MUSCLE FIBERS AFTER HEAVY EXERCISE
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DOI：
10.1152/jappl.1973.34.5.615
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发表时间：
1973-01-01
影响因子：
3.3
通讯作者：
SALTIN, B
中科院分区：
文献类型：
--
作者：
GOLLNICK, PD;ARMSTRONG, RB;SALTIN, B
首页--期刊主要分类--期刊细介绍--期刊题录与文摘--期刊详细文摘内容大负荷运动后人体骨骼肌糖原耗竭模式。J.Appl.物理。34(5)：6 15-618。1973年-在六名受试者的短时间高强度运动中，观察了人体骨骼肌纤维中糖原耗竭的模式。锻炼包括以每分钟90或104转的速度骑自行车测功器，以及计算出的阻力，其能量消耗是受试者有氧能力的150%。进行六次i-min冲刺，每次运动之间有10分钟的休息时间。在第一次、第三次和最后一次训练后，从静息状态下的股外侧肌取活组织标本。肌肉总糖原随着训练次数的增加而下降。根据PAS染色强度用组织化学方法估计肌纤维中糖原的相对浓度。最先耗尽其糖原储存的纤维是低氧化、高糖酵解、快速抽动的纤维。这表明，在大强度运动中，这些纤维可能会及早被招募。这种模式与长时间、中等强度运动时看到的情况形成了鲜明对比，当时高氧化的慢抽动纤维首先耗尽了它们的糖原储备。血乳酸；肌糖原；大运动量；肌肉纤维利用组织化学糖原耗竭模式表明，人类骨骼肌的慢抽动(ST)纤维优先用于长时间、中等强度的运动(未发表的观察结果)。当ST纤维的糖原耗尽时，快速抽动(FT)纤维逐渐被招募。相反，在电刺激产生的最大肌肉收缩中，大鼠和豚鼠骨骼肌的快速抽动白纤维最先变为磷酸化酶阴性，并耗尽其糖原储存(4，9)。问题是，人类肌肉的FT纤维是否在高强度运动的早期阶段得到了利用。由于FT纤维的氧化能力一般低于ST纤维(5)，同时使用这两种类型的纤维会导致这些纤维的糖原储备提前耗尽。这项研究的目的是在自行车运动中跟踪人体骨骼肌快抽动和慢抽动纤维的糖原耗竭模式，自行车运动需要产生相当于受试者有氧能力150%的能量。根据这些数据，得出了关于这两种纤维类型在高强度短期运动中的招募的结论。
GOLLNICK, PD, RB ARMSTRONG, WL SEMBROWICH, RE SHEPHERD, AND B. SALTIN. Glycogen depletion pattern in human skeletal muscle jibers after heavy exercise. J. Appl. Physiol. 34 (5): 6 15-618. 1973.-The glycogen depletion pattern in human skeletal muscle fibers was followed during short high-intensity work in six subjects. Exercise consisted of pedaling a bicycle ergometer at 90 or 104 rpm and a resistance calculated to require an energy expenditure of 150% of the subject’s aerobic power. Six I-min sprints were performed with lo-min rest periods between each exercise. Biopsy samples were taken from the vastus lateralis muscle at rest and after the first, third, and final work bouts. Total muscle glycogen declined as a function of the number of work bouts. The relative concentration of glycogen in the muscle fibers was histochemically estimated from PAS staining intensity. The first fibers to become depleted of their glycogen stores were the low-oxidative, high-glycolytic, fast twitch fibers. This suggests an early recruitment of these fibers during heavy exercise. This pattern is in contrast to that seen during prolonged, moderately-intense exercise when the high-oxidative slow twitch fibers are the first to become depleted of their glycogen reserves. blood lactate; muscle glycogen; heavy work; muscle fiber utilizationHISTOCHEMICAL GLYCOGEN DEPLETION patterns indicate that the slow twitch (ST) fibers of human skeletal muscle are preferentially used in prolonged, moderately-intense exercise (unpublished observations). Fast twitch (FT) fibers are progressively recruited as the glycogen of the ST fibers becomes depleted. In contrast, during maximal muscular contraction produced by electrical stimulation the fasttwitch white fibers of rat and guinea pig skeletal muscle are the first to become phosphorylase negative and depleted of their glycogen stores (4, 9). The question arises as to whether or not the FT fibers of human muscle are utilized in the early stages of high-intensity exercise. Since FT fibers generally have lower oxidative capacity than ST fibers (5) the simultaneous use of both fiber types would result in an early depletion of the glycogen reserves of these fibers. The purpose of this study was to follow the glycogen depletion pattern in fast-and slow-twitch fibers of human skeletal muscle during bicycle exercise requiring an energy production equivalent to 150% of the subject’s aerobic power. From these data conclusions have been drawn concerning the recruitment of the two fiber types during highintensity, short-term exercise.