SdsA1, a secreted sulfatase, contributes to the in vivo virulence of Pseudomonas aeruginosa in mice.
SdsA1, a secreted sulfatase, contributes to the in vivo virulence of Pseudomonas aeruginosa in mice.
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SdsA1 是一种分泌型硫酸酯酶，有助于铜绿假单胞菌在小鼠体内的毒力。
DOI：
10.1111/1348-0421.12772
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Koichi Kuwano
中科院分区：
文献类型：
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作者：
Yutaka Kida;Takeshi Yamamoto;Koichi Kuwano
粘蛋白是一种糖蛋白，是覆盖上皮组织的粘液的主要成分。由于粘蛋白作为先天免疫系统的第一线发挥作用，铜绿假单胞菌似乎需要与粘蛋白相互作用才能在宿主中建立感染。然而，P. aerobiosa和粘蛋白的研究还很少。在这项研究中，使用体内表达技术（IVET），我们试图确定粘蛋白诱导的启动子，可能参与建立P。呼吸道感染IVET分析显示，编码被认为是利用粘蛋白作为营养物所需的糖苷酶、硫酸酯酶和肽酶的基因存在于所鉴定的启动子下游的13个基因中。我们的研究结果表明，其中，sdsA1编码分泌硫酸酯酶起着核心作用，在降解粘蛋白。然后证明，sdsA1的破坏导致硫酸盐从粘蛋白和硫酸化糖的释放减少。此外，与野生型菌株相比，dsA1突变体在白细胞减少小鼠中显示粘蛋白凝胶穿透能力降低和毒力减弱。这些结果表明，SdsA1作为P.铜绿。
Mucin is a glycoprotein that is the primary component of the mucus overlaying the epithelial tissues. Because mucin functions as a first line of the innate immune system,Pseudomonas aeruginosaappears to require interaction with mucin to establish infection in the host. However, the interactions betweenP. aeruginosaand mucin have been poorly understood. In this study, using in vivo expression technology (IVET), we attempted to identify mucin‐inducible promoters that are likely to be involved in the establishment ofP. aeruginosainfection. The IVET analysis revealed that the genes encoding glycosidases, sulfatases, and peptidases that are thought to be required for the utilization of mucin as a nutrient are present in 13 genes downstream of the identified promoters. Our results indicated that, among them,sdsA1encoding a secreted sulfatase plays a central role in the degradation of mucin. It was then demonstrated that disruption ofsdsA1leads to a decreased release of sulfate from mucin and sulfated sugars. Furthermore, thesdsA1mutant showed a reduction in the ability of mucin gel penetration and an attenuation of virulence in leukopenic mice compared with the wild‐type strain. Collectively, these results suggest that SdsA1 plays an important role as a virulence factor ofP. aeruginosa.