PRQFVamide, a novel pentapeptide identified from the CNS and gut of Aplysia
PRQFVamide, a novel pentapeptide identified from the CNS and gut of Aplysia
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DOI：
10.1152/jn.00014.2003
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发表时间：
2003-06-01
影响因子：
2.5
通讯作者：
Vilim, FS
中科院分区：
文献类型：
--
作者：
Furukawa, Y;Nakamaru, K;Vilim, FS
我们利用对肠道收缩的生物活性测定，从阿龙鱼神经系统中纯化了一种新的五肽。肽的结构是Pro-Arg-Gln-Phe-Val-酰胺（PRQFVa）。发现PRQFVa的前体编码33个拷贝的PRQFVa酰胺和4个相关的五肽。通过基质辅助激光解吸/电离飞行时间质谱法在Aaplasia神经元中检测到与所有五种五肽的预测质量相对应的峰。北方分析显示前体蛋白在腹神经节中表达丰富，在足神经节和大脑神经节中表达较少，在颊神经节和胸膜神经节中很少见到。PRQFVa阳性神经元，映射通过免疫组织化学和原位杂交，存在于所有的中央神经节。在肠道中观察到PRQFVa免疫阳性过程，特别是与脉管系统相关。一些动脉和其他高度血管化的组织，如鳃和肾，也含有许多PRQFVa免疫阳性过程。合成PRQFVa的应用不仅抑制肠的收缩，而且抑制脉管系统的收缩。PRQFVa在进食回路内的一些神经元中表达，并且发现合成的PRQFVa的应用降低了颊部进食回路的一些（B4/5和B31/32）但不是所有（B8）神经元的兴奋性。我们的研究结果表明，PRQFVa可能作为一个调制器内的喂养系统以及在其他系统的失智症。
We have purified a novel pentapeptide from the Aplysia nervous system using bioassay on gut contractions. The structure of the peptide is Pro-Arg-Gln-Phe-Val-amide (PRQFVa). The precursor for PRQFVa was found to code for 33 copies of PRQFVamide and four related pentapeptides. Peaks corresponding to the predicted masses of all five pentapeptides were detected in Aplysia neurons by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Northern analysis revealed that expression of the precursor is abundant in the abdominal ganglion, much less in the pedal and cerebral ganglia, and rarely seen in the buccal and pleural ganglia. PRQFVa-positive neurons, mapped by immunohistochemistry and in situ hybridization, were present in all the central ganglia. PRQFVa immunopositive processes were observed in the gut, particularly in association with the vasculature. Some arteries and other highly vascularized tissues, such as the gill and the kidney, also contain numerous PRQFVa immunopositive processes. Application of synthetic PRQFVa suppresses not only contractions of the gut but also contractions of vasculature. PRQFVa is expressed in some of the neurons within the feeding circuitry and application of synthetic PRQFVa was found to decrease the excitability of some (B4/5 and B31/32) but not all (B8) neurons of the buccal feeding circuit. Our findings suggest that PRQFVa may act as a modulator within the feeding system as well as in other systems of Aplysia.