Effect of diet on fat cell size and hormone-sensitive lipase activity
Effect of diet on fat cell size and hormone-sensitive lipase activity
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DOI：
10.1152/jappl.1999.87.1.227
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发表时间：
1999-07-01
影响因子：
3.3
通讯作者：
Barnard, RJ
中科院分区：
文献类型：
--
作者：
Berger, JJ;Barnard, RJ
饮食对脂肪细胞大小和激素敏感脂肪酶活性的影响。 J.应用程序。生理学。 87（1）。 227-232, 1999.-这项研究旨在检查饮食引起的胰岛素抵抗/高胰岛素血症、脂肪细胞肥大和激素敏感性脂肪酶 (HSL) 之间的关系，以阐明 HSL 活性减弱是否会导致肥胖。雌性 Fischer 344 大鼠喂食低脂肪、复杂碳水化合物饮食或高脂肪、精制糖 (HFS) 饮食，持续 2 周、2 个月或 6 个月。在这些时间测定脂肪酶组织形态和HSL活性以及血浆游离脂肪酸和甘油水平。 2 周后，除了先前报道的 HFS 动物出现高胰岛素血症外，组间没有发现差异。在 2 个月和 6 个月时，HFS 动物均表现出脂肪细胞肥大。 HFS 组的基础和刺激 HSL 活性以及血浆甘油显着升高。基础状态和刺激状态下脂肪细胞大小与 HSL 活性 Ebr 呈正相关。这些结果证明，在胰岛素抵抗/高胰岛素血症发作时没有观察到HSL活性减弱，因此在肥胖的发展中不发挥作用。
Effect of diet on fat cell size and hormone-sensitive lipase activity. J.Appl. Physiol. 87(1). 227-232, 1999.-This study was designed to examine the relationship between diet-induced insulin resistance/hyperinsulinemia, fat cell hypertrophy, and hormone-sensitive lipase (HSL) to elucidate whether an attenuated HSL activity leads to obesity. Female Fischer 344 rats were fed either a low-fat, complex-carbohydrate diet or a high-fat, refined-sugar (HFS) diet for 2 wk, 2 mo, or 6 mo. Adipase tissue morphology and HSL activity as well as plasma free fatty acid and glycerol levels were determined at these times. No differences between groups were seen after 2 wk except the previously reported hyperinsulinemia in the HFS animals. At both 2 and 6 mo, the HFS animals demonstrated adipocyte hypertrophy. Basal and stimulated HSL activities and plasma glycerol were significantly elevated in the HFS group. There was a positive correlation between adipocyte size and HSL activity Ebr both basal and stimulated states. These results demonstrate that an attenuated HSL activity is not observed with the onset of insulin resistance/hyperinsulinemia and therefore does not play a role in the development of obesity.