Solid-phase extraction based on a molecularly imprinted polymer nanoshell at the surface of silica nanospheres for the specific enrichment and identification of alkaloids from Crinum asiaticum L. var. sinicum
Solid-phase extraction based on a molecularly imprinted polymer nanoshell at the surface of silica nanospheres for the specific enrichment and identification of alkaloids from Crinum asiaticum L. var. sinicum
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基于二氧化硅纳米球表面分子印迹聚合物纳米壳的固相萃取，用于特定富集和鉴定文殊兰生物碱。
DOI：
10.1002/jssc.201601116
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发表时间：
2016
影响因子：
3.1
通讯作者：
Yue
中科院分区：
文献类型：
--
作者：
Yang;Ruixiang;Zhu;Dong;Wen;Hongmei;Fu;Anchen;Zhao;Zihan;Dai;Guoying;Miao;Zhaoyi;Hu;Yue
制备了二氧化硅纳米球表面的分子印迹纳米壳，用于文殊兰生物碱的特异性富集和鉴定。中华。以石蒜碱为模板、丙烯酰胺为功能单体、乙二醇二甲基丙烯酸酯为交联剂、2′,2-偶氮二异丁腈为引发剂、乙腈为成孔剂，通过表面聚合法合成纳米壳。通过透射电子显微镜和红外光谱对核壳纳米球进行了表征，结果表明纳米壳层均匀地附着在乙烯基改性的SiO2纳米球的表面。通过结合平衡和吸附动力学实验估计纳米球的吸附能力。纳米球对石蒜碱的最大吸附量为6.68 μmol/g，印迹因子接近2.5，印迹效果良好。该纳米球成功应用于固相萃取文殊兰中的石蒜碱。大鼠代谢物中靶分子的sinicumand检测。 Crinum asaticum L. var. 中石蒜碱的平均回收率紫草提取物和大鼠代谢物分别为 93.5 ± 0.6% (n= 3) 和 91.6 ± 1.9% (n= 3)。这项工作为在基于二氧化硅纳米球的固相萃取用于药物分析的表面制造分子印迹纳米壳提供了一种简单的方法。
A molecularly imprinted nanoshell on the surface of silica nanospheres was prepared for specific enrichment and identification of alkaloids fromCrinum asiaticum L. var. sinicum. The nanoshell was synthesized by surface polymerization using lycorine as the template, acrylamide as the functional monomer, ethylene glycol dimethacrylate as the cross‐linker, 2′,2‐azobisisobutyronitrile as the initiator and acetonitrile as the pore‐forming agent. The core–shell nanospheres were characterized by transmission electron microscopy and infrared spectroscopy, and the results show that the nanoshell layer was homogeneously attached to the surface of vinyl‐modified SiO2nanospheres. The adsorption capacity of the nanospheres was estimated by binding equilibrium and adsorption kinetics experiments. The maximum adsorption amount of lycorine on the nanospheres was 6.68 μmol/g and the imprinting factor was nearly 2.5, indicating a good imprinting effect. The nanospheres were successfully applied in solid‐phase extraction for lycorine fromCrinum asaticum L. var. sinicumand detection of target molecule in rat metabolites. The average recoveries of lycorine inCrinum asaticum L. var. sinicumextraction and rat metabolites were 93.5 ± 0.6% (n= 3) and 91.6 ± 1.9% (n= 3), respectively. This work provides a simple approach for the fabrication of a molecularly imprinted nanoshell at the surface of silica nanospheres‐based solid‐phase extraction for drug analysis.