Distribution of members of the PSD-95 family of MAGUK proteins at the synaptic region of inner and outer hair cells of the guinea pig cochlea
Distribution of members of the PSD-95 family of MAGUK proteins at the synaptic region of inner and outer hair cells of the guinea pig cochlea
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DOI：
10.1002/syn.1048
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发表时间：
2001-06-15
期刊：
影响因子：
2.3
通讯作者：
Petralia, RS
中科院分区：
文献类型：
--
作者：
Davies, C;Tingley, D;Petralia, RS
PDZ结构域包含MAGUK(膜相关鸟苷酸激酶)家族的蛋白，靶向、锚定和聚集受体和通向亚细胞位置的通道。在Maguk蛋白中，PSD-95家族的成员(MAGUKs：PSD-95、PSD-93、SAP-97和SAP-102)靶向并将谷氨酸受体锚定到突触终末。谷氨酸受体与MAGUK之间的联系已经在大脑中被描述，但在耳蜗中还没有。本研究利用RT-PCR、免疫荧光显微镜和免疫电子显微镜研究了Maguk蛋白在Corti器官中的存在和分布。RT-PCR证实PSD-95、PSD-93、SAP-97和SAP-102的信使核糖核酸在Corti器官中存在，并使用可识别所有四种MAGUK蛋白的“PAN-MAGUK”抗体进行免疫细胞化学，针对这些蛋白的选择性抗体显示，所有四种MAGUK都存在于内毛细胞的底部，而除SAP-97外的所有MAGUK都存在于外毛细胞的底部。此外，PSD-93和PSD-95存在于内毛细胞的突触后传入终末，而在外毛细胞的突触后传入终末有PSD-93。Synapse 40：258-268,2001，(C)2001 Wiley-Liss，Inc.Dagger
PDZ-domain containing proteins of the MAGUK (membrane-associated guanylate kinase) family target, anchor, and cluster receptors and channels to subcellular sites. Among the MAGUK proteins, the members of the PSD-95 family (MAGUKs: PSD-95, PSD-93, SAP-97, and SAP-102) target and anchor glutamate receptors to the synaptic terminals. Associations of glutamate receptors with MAGUKs have been described in the brain but not in the cochlea. In this study, RT-PCR, immunofluorescence microscopy, and immunoelectron microscopy were used to investigate the presence and distribution of MAGUK proteins in the organ of Corti. The presence of the mRNA for PSD-95, PSD-93, SAP-97, and SAP-102 in the organ of Corti was confirmed by RT-PCR, Immunocytochemistry using a "pan-MAGUK" antibody, which recognizes all four MAGUK proteins, and selective antibodies against these proteins revealed that all four MAGUKs are present within the base of inner hair cells while all except SAP-97 are found within the base of the outer hair cells. In addition, PSD-93 and PSD-95 are found in postsynaptic afferent terminals on inner hair cells, while postsynaptic afferent terminals on outer hair cells have PSD-93. Synapse 40:258-268, 2001, (C) 2001 Wiley-Liss, Inc.dagger