The cell surface-associated intercellular C-signal induces behavioral changes in individual Myxococcus xanthus cells during fruiting body morphogenesis
The cell surface-associated intercellular C-signal induces behavioral changes in individual Myxococcus xanthus cells during fruiting body morphogenesis
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DOI：
10.1073/pnas.96.9.5031
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发表时间：
1999-04-27
影响因子：
11.1
通讯作者：
Sogaard-Andersen, L
中科院分区：
文献类型：
--
作者：
Jelsbak, L;Sogaard-Andersen, L
黄色粘球菌子实体的形成依赖于细胞在饥饿条件下从群集到聚集的有序变化。我们表明，适当饥饿的个体细胞在子实体形成过程中改变行为。具体而言，从聚集开始时起，单个野生型细胞开始以增加的滑动速度移动，平均滑动间隔的持续时间增加，并且停止频率降低，而平均停止间隔的持续时间和逆转频率保持不变。缺乏细胞表面相关的细胞间C信号的突变体（csgA突变体）未能聚集。同样，适当饥饿的单个csgA细胞在发育过程中也不会改变它们的行为。在没有…的情况下;其他细胞-细胞相互作用，个别csgA细胞的运动缺陷，纠正后，外源性MalE-CsgA蛋白C-信号重建的时间和浓度依赖性的方式。C-信号刺激运动依赖于胞质Frz信号转导系统。我们认为，C-信号指示细胞在发育过程中以高速度和低停止和反转频率移动到聚集中心。
Fruiting body formation in Myxococcus xanthus depends on ordered changes in cell movements from swarming to aggregation in response to starvation. We show that appropriately starved individual cells change behavior during fruiting body formation. Specifically, from the time of initiation of aggregation, individual wild-type cells began to move with increased gliding speeds, the duration of the mean gliding interval increased, and the stop frequency decreased whereas the duration of the mean stop interval and the reversal frequency remained unchanged. Mutants lacking the cell surface-associated, intercellular C-signal (csgA mutants) failed to aggregate. Likewise, appropriately starved individual csgA cells did not change their behavior during development. In the absence of;other cell-cell interactions, the motility defect of individual csgA cells was corrected in a time- and concentration-dependent manner after C-signaling was reestablished by exogenous MalE-CsgA protein. The C-signal-induced stimulation of motility depended on the cytoplasmic Frz signal transduction system. We propose that C-signal instructs cells to move with high speed and low stop and reversal frequencies into aggregation centers during development.