Sperm binding to eggs of Ciona intestinalis. Role of Ca2+.
Sperm binding to eggs of Ciona intestinalis. Role of Ca2+.
复制标题
精子与海鞘卵子结合。
DOI：
10.1016/0014-4827(84)90787-0
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发表时间：
1984
影响因子：
3.7
通讯作者：
M. Pinto
中科院分区：
文献类型：
--
作者：
G. Casazza;R. Santis;M. Pinto
在本文中，我们表明，在ascidiaCiona tuminalis细胞外Ca 2+是必需的精子的结合卵黄涂层（VC）甘油处理的鸡蛋和受精发生。二价阳离子Mg 2+和Mn 2+不能取代Ca 2+。一旦结合，精子就不能通过加入EGTA从卵黄膜上脱离。维拉帕米不干扰精子与卵黄膜的结合，但它能阻断Ca ~（2+）离子载体A23187诱导的精子活化和顶体反应。这种药物的存在也抑制了受精。
In this paper we show that in the ascidiaCiona intestinalisextracellular Ca2+is required for the binding of the spermatozoa to the vitelline coat (VC) glycerol-treated eggs and for fertilization to occur. Divalent cations, Mg2+and Mn2+, cannot replace Ca2+. Once bound, the spermatozoa cannot be detached from the vitelline coat by adding of EGTA. Verapamil does not interfere with the binding of spermatozoa to the vitelline coat, whereas it blocks the Ca2+ionophore A23187-induced sperm activation and acrosome reaction. Fertilization too was inhibited by the presence of this drug.