Effects of hemodilution with a hemoglobin-based oxygen carrier (HBOC-201) on ischemia/reperfusion injury in a model of partial warm liver ischemia of the rat
Effects of hemodilution with a hemoglobin-based oxygen carrier (HBOC-201) on ischemia/reperfusion injury in a model of partial warm liver ischemia of the rat
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DOI：
10.1016/j.mvr.2009.08.005
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发表时间：
2009-12-01
影响因子：
3.1
通讯作者：
Golling, Markus
中科院分区：
文献类型：
--
作者：
Zapletal, Christina;Bode, Alexander;Golling, Markus
背景：缺血/再灌注损伤是肝脏手术和移植中不可避免的并发症。胶体血液稀释可减少缺血后损伤，但限制氧转运。基于血红蛋白的氧载体已被评估为血液替代品，并提供血浆来源的氧运输。我们研究的目的是评价血液稀释与使用HBOC-201（Hemopure（R））为再灌注肝组织提供更好氧气供应的综合效益。材料与方法：采用大鼠部分热肝缺血模型。一组作为未处理的对照，其他组用乳酸林格氏液、右旋糖酐-70、HBOC-201或右旋糖酐和HBOC-201的混合物进行血液稀释。再灌注后，进行活体显微镜研究，并测量组织pO（2）水平和转氨酶。通过单因素和双因素ANOVA进行统计分析，然后进行成对比较。结果：与对照组相比，乳酸林格氏液血液稀释未显示出任何改善。在研究的所有参数中，右旋糖酐和HBOC组均上级林格氏液和对照动物。窦后微静脉中白细胞粘附从对照组和Ringer组的569.03 +/- 171.87和364.52 +/- 167.32增加到右旋糖酐和HBOC组的131.68 +/- 58.34和68.44 +/- 20.31/mm（2）内皮细胞（p
Background: Ischemia/reperfusion injury is an unavoidable complication in liver surgery and transplantation. Hemodilution with colloids can reduce postischemic injury but limits oxygen transport. Hemoglobin-based oxygen carriers have been evaluated as blood substitute and provide a plasma-derived oxygen transport. It was the aim of our study to evaluate the combined benefits of hemodilution with a better oxygen supply to reperfused liver tissue by the use of HBOC-201 (Hemopure (R)). Material and methods: A model of partial warm liver Ischemia in the rat was used. One group served as untreated control, the other groups were hemodiluted either with Ringer's lactate, Dextran-70, HBOC-201 or a mixture of Dextran and HBOC-201. After reperfusion, intravital microscopy studies were done and tissue pO(2) levels and transaminases measured. Statistical analysis was done by one- and two-way ANOVA, followed by pairwise comparison. Results: Hemodilution with Ringer's lactate did not show any improvement compared to the control group. Dextran and HBOC group were superior to the Ringer and control animals in all parameters studied. Leucocyte adherence in postsinusoidal venules improved from 569.03 +/- 171.87 and 364.52 +/- 167.32 in control and Ringer group to 131.68 +/- 58.34 and 68.44 +/- 20.31/mm(2) endothelium in Dextran and HBOC group (p