Multifunctional Ca2+/calmodulin-dependent protein kinase is necessary for nuclear envelope breakdown.
Multifunctional Ca2+/calmodulin-dependent protein kinase is necessary for nuclear envelope breakdown.
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DOI：
10.1083/jcb.111.5.1763
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发表时间：
1990-11
期刊：
影响因子：
--
通讯作者：
Steinhardt RA
中科院分区：
文献类型：
--
作者：
Baitinger C;Alderton J;Poenie M;Schulman H;Steinhardt RA
研究了多功能钙/钙调蛋白依赖的蛋白激酶(CaM)在海胆卵核膜破裂(NEB)中的作用。这些卵子含有一种56kD的多肽，似乎是神经元CaM激酶的同源物。例如，它经历了依赖于钙离子/钙调蛋白的自动磷酸化，将其转化为一个不依赖于钙离子的物种，这是多功能CaM激酶的标志。它与大鼠脑钙调素激酶的α亚基同源。在体外，CaMK(273-302)抑制了海胆卵蛋白激酶的自磷酸化和底物磷酸化，CaMK(273-302)是一种与神经元CaM激酶的自身抑制域相对应的合成肽。当显微注射到海胆卵中时，这种多肽抑制NEB。只有一株抗神经元酶的单抗免疫沉淀了56-kD的多肽。当显微注射到海胆卵中时，只有这种抗体才能阻断或显著延迟NEB。这些结果表明，海胆卵中含有多功能的CaM激酶，该酶参与了有丝分裂过程中NEB的调控。
The role of multifunctional Ca2+/calmodulin-dependent protein kinase (CaM kinase) in nuclear envelope breakdown (NEB) was investigated in sea urchin eggs. The eggs contain a 56-kD polypeptide which appears to be a homologue of neuronal CaM kinase. For example, it undergoes Ca2+/calmodulin-dependent autophosphorylation that converts it to a Ca2(+)-independent species, a hallmark of multifunctional CaM kinase. It is homologous to the alpha subunit of rat brain CaM kinase. Autophosphorylation and substrate phosphorylation by the sea urchin egg kinase are inhibited in vitro by CaMK(273-302), a synthetic peptide corresponding to the autoinhibitory domain of the neuronal CaM kinase. This peptide inhibited NEB when microinjected into sea urchin eggs. Only one mAb to the neuronal enzyme immunoprecipitated the 56-kD polypeptide. Only this antibody blocked or significantly delayed NEB when microinjected into sea urchin eggs. These results suggest that sea urchin eggs contain multifunctional CaM kinase, and that this enzyme is involved in the control of NEB during mitotic division.