Peptide templates for nanoparticle synthesis derived from polymerase chain reaction-driven phage display
Peptide templates for nanoparticle synthesis derived from polymerase chain reaction-driven phage display
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DOI：
10.1002/adfm.200304501
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发表时间：
2004-01-01
影响因子：
19
通讯作者：
Stone, MO
中科院分区：
文献类型：
--
作者：
Naik, RR;Jones, SE;Stone, MO
噬菌体肽展示库通常用于选择与无机表面（金属、金属氧化物和半导体）结合的肽。这些结合肽可以作为模板来控制体外无机纳米颗粒的成核和生长。在本报告中，我们描述了使用聚合酶链反应（PCR）驱动的方法从噬菌体肽展示库中鉴定出一组与银和钴纳米粒子结合的独特序列。通过 PCR 方法获得的氨基酸序列是一组独特的序列，否则使用常规淘选方法会丢失这些序列。通过此处描述的方法鉴定的肽也被证明可以作为合成银和钴铂纳米颗粒的模板。
Phage peptide display libraries are commonly used to select peptides that bind to inorganic surfaces (metals, metal oxides, and semiconductors). These binding peptides can serve as templates to control the nucleation and growth of inorganic nanoparticles in vitro. In this report, we describe the identification of a unique set of sequences that bind to silver and cobalt nanoparticles from a phage peptide display library using a polymerase chain reaction (PCR)-driven method. The amino acid sequences obtained by the PCR method are a distinct set of sequences that would otherwise be missed using the regular panning method. Peptides identified by the method described here are also shown to function as templates for the synthesis of silver and cobalt platinum nanoparticles.