Preferential expansion of autoreactive T lymphocytes from the memory T-cell pool by IL-7
Preferential expansion of autoreactive T lymphocytes from the memory T-cell pool by IL-7
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DOI：
10.1016/s0165-5728(99)00200-3
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发表时间：
1999-12-01
影响因子：
3.3
通讯作者：
Martin, R
中科院分区：
文献类型：
--
作者：
Bielekova, B;Muraro, PA;Martin, R
我们已经开发了一种新的技术，使我们能够量化抗原特异性T细胞，并确定其功能表型和起源从幼稚与记忆人口。使用这种方法，我们已经确定了共有286个T细胞系特异性髓鞘碱性蛋白（MBP）和流感血凝素从16多发性硬化症（MS）患者和9名健康供体。我们的数据支持这样的观点，即MBP特异性T细胞在MS患者体内活化，并表明存在免疫失调，使MS患者易于发展自身免疫。我们的方法提供了一种研究抗原特异性T细胞特征作为免疫治疗试验中的替代标志物的方法。(C)1999 Elsevier Science B.V.保留所有权利。
We have developed a new technique that allows us to quantify antigen-specific T cells, and to determine their functional phenotype and origin from naive versus memory populations. Using this methodology, we have characterized a total of 286 T-cell lines specific for myelin basic protein (MBP) and influenza hemagglutinin from 16 multiple sclerosis (MS) patients and nine healthy donors. Our data support the notion that MBP-specific T cells undergo in vivo activation in MS patients and indicate a presence of immune dysregulation that renders MS patients prone to develop autoimmunity. Our methodology offers a way to study antigen-specific T-cell characteristics as a surrogate marker in immunotherapy trials. (C) 1999 Elsevier Science B.V. All rights reserved.