Primary structure of rat hepatocyte growth factor and induction of its mRNA during liver regeneration following hepatic injury.
Primary structure of rat hepatocyte growth factor and induction of its mRNA during liver regeneration following hepatic injury.
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大鼠肝细胞生长因子的一级结构及其 mRNA 在肝损伤后肝再生过程中的诱导。
DOI：
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Naomi Kitamura
中科院分区：
文献类型：
--
作者：
A. Okajima;Keiji Miyazawa;Naomi Kitamura
将克隆的人肝细胞生长因子（hHGF） cDNA与大鼠肝细胞生长因子（rHGF） cDNA进行交叉杂交，分离得到重叠cDNA克隆，并测定其核苷酸序列。根据该序列推导出了rHGF的整个一级结构。比较大鼠和人HGFs之间的氨基酸序列发现，这两个序列在整个蛋白质结构中都高度保守，表明大鼠和人HGFs可能在功能上相似。结合rHGF cDNA探针，采用Northern blot杂交分析rHGF mRNA对大鼠肝脏再生的影响。四氯化碳给药后，肝脏和脾脏mRNA水平升高，而肾脏mRNA水平无升高。在最大诱导水平下，肝脏中rHGF mRNA比正常水平增加约4.5倍。d -半乳糖胺处理后，肝脏和脾脏mRNA水平升高。另一方面，肝部分切除后肝脏和脾脏中mRNA未见明显升高。这些观察结果表明，HGF可能是肝毒素引起的肝损伤后肝脏再生的调节剂。
Overlapping cDNA clones for rat hepatocyte growth factor (rHGF) were isolated by cross-hybridization with the cloned cDNA for human hepatocyte growth factor (hHGF) and the nucleotide sequence of the cDNA was determined. The entire primary structure of rHGF was deduced from the sequence. Comparison of the amino acid sequences between rat and human HGFs revealed that the two sequences are highly conserved throughout the protein structures, suggesting that rat and human HGFs may be functionally similar. Responses of the rHGF mRNA during liver regeneration in rats were examined by Northern blot hybridization analysis with the aid of the cDNA probe for rHGF. The mRNA levels increased in the liver and spleen but not in the kidney after administration of carbon tetrachloride. At the maximum level of induction, the rHGF mRNA increased in the liver about 4.5-fold over its normal level. The mRNA levels also increased in the liver and spleen after administration of D-galactosamine. On the other hand, no obvious increase of the mRNA was observed in the liver and spleen after partial hepatectomy. These observations suggest that HGF may function as a regulator of liver regeneration following hepatic injury caused by hepatotoxins.
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作者：
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DOI：
--
发表时间：
1982
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
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DOI：
10.1073/pnas.86.19.7432
发表时间：
1989-10-01
影响因子：
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作者：
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影响因子：
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作者：
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通讯作者：
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