Electrophoretic Patterns of Extracellular Deoxyribonuclease (DNase) and Their Correlation with T‐Type in Group A Streptococci
Electrophoretic Patterns of Extracellular Deoxyribonuclease (DNase) and Their Correlation with T‐Type in Group A Streptococci
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A 族链球菌胞外脱氧核糖核酸酶 (DNase) 电泳图谱及其与 T 型的相关性
DOI：
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发表时间：
1985
影响因子：
2.6
通讯作者：
Y. Fukazawa
中科院分区：
文献类型：
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作者：
Y. Miyakawa;Toshihiko Yamada;M. Shitara;Y. Fukazawa
A 组链球菌胞外脱氧核糖核酸酶 (DNase) 的分子异质性在 42 个临床分离株中得到证实。尽管所有分离株胞外 DNase 的聚丙烯酰胺凝胶电泳图谱存在异质性，但根据是否存在包括 DNase B 在内的三种 DNase 成分，可将它们分为 5 种主要模式。通过将所有分离株的 DNase 电泳图谱与其 T 型进行比较，我们发现这些分离株的电泳图谱非常具有 T 型的特征，特别是在流行的 T 型 12 和 1 中，并且 T 型的分离株12 和 1 产生 DNase B 作为其主要的细胞外 DNase。通过抗 DNase B 抗体快速中和法测定 A、B 和 G 组分离株的总细胞外 DNase 活性中的相对 DNase B 活性。结果显示，A 组链球菌的所有分离株中的 DNase 活性均被中和，很大程度上与其 T 型相对应，但 B 组和 G 组的分离株则不然。这些结果表明，A 组链球菌胞外 DNase 的电泳模式与其 T 型密切相关，表明这些特性的理化分类学价值。
Molecular heterogeneity of the extracellular deoxyribonuclease (DNase) in group A streptococci was demonstrated in 42 clinical isolates. Although Polyacrylamide gel electrophoretic patterns of the extracellular DNase of all the isolates were heterogeneous, they could be divided into five main patterns with respect to the presence or absence of three DNase components including DNase B. By comparing the electrophoretic patterns of DNase in all the isolates with their T‐types, we found that the patterns were quite characteristic for their T‐types, especially in the prevalent T‐types 12 and 1, and that the isolates of T‐types 12 and 1 produced DNase B as their major extracellular DNase. Relative DNase B activity in the total extracellular DNase activity of group A, B, and G isolates was determined by the rapid method of neutralization with anti‐DNase B antibody. The results showed neutralization of DNase activity in all the isolates of group A streptococci, largely corresponding to their T‐types, but not of the isolates of groups B and G. These results indicate that the electrophoretic patterns of the extracellular DNase of group A streptococci are closely correlated with their T‐types, suggesting the physicochemical taxonomic value of these properties.