Stable expression of rat cytochrome P-450IIB1 cDNA in Chinese hamster cells (V79) and metabolic activation of aflatoxin B1.
Stable expression of rat cytochrome P-450IIB1 cDNA in Chinese hamster cells (V79) and metabolic activation of aflatoxin B1.
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大鼠细胞色素 P-450IIB1 cDNA 在中国仓鼠细胞 (V79) 中的稳定表达和黄曲霉毒素 B1 的代谢激活。
DOI：
10.1073/pnas.85.16.5769
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发表时间：
1988
影响因子：
11.1
通讯作者：
Oesch,F
中科院分区：
文献类型：
--
作者：
Doehmer,J;Dogra,S;Friedberg,T;Monier,S;Adesnik,M;Glatt,H;Oesch,F
中国仓鼠成纤维细胞被广泛用于致突变性试验，但存在严重的局限性，即它们不表达细胞色素P-450，而细胞色素P-450是激活许多促突变性代谢物所需的细胞色素。构建了猴病毒40早期启动子控制的单加氧酶细胞色素P-450IIB1全长cDNA克隆，并与选择标记新霉素磷酸转移酶（赋予G418抗性）共导入V79中国仓鼠细胞。选择抗g418细胞，建立细胞系，检测细胞色素P-450IIB1的表达和酶活性。发现两个细胞系（SD1和SD3）稳定产生细胞色素P-450IIB1。虽然纯化的细胞色素P-450只有在与细胞色素P-450还原酶和磷脂重组后才具有单加氧酶活性，但该结构的基因产物显示出这种活性。这意味着基因产物以一种允许获得所需成分的方式定位于细胞内。与V79细胞相比，暴露于黄曲霉毒素B1后，SD1细胞的次黄嘌呤磷酸化酰基转移酶（HPRT）位点的突变率显著增加。
V79 Chinese hamster fibroblasts are widely used for mutagenicity testing but have the serious limitation that they do not express cytochromes P-450, which are needed for the activation of many promutagens to mutagenic metabolites. A full-length cDNA clone encoding the monooxygenase cytochrome P-450IIB1 under control of the simian virus 40 early promoter was constructed and cointroduced with the selection marker neomycin phosphotransferase (conferring resistance to G418) into V79 Chinese hamster cells. G418-resistant cells were selected, established as cell lines, and tested for cytochrome P-450IIB1 expression and enzymatic activity. Two cell lines (SD1 and SD3) were found that stably produce cytochrome P-450IIB1. Although purified cytochromes P-450 possess monooxygenase activity only after reconstitution with cytochrome P-450 reductase and phospholipid, the gene product of the construct exhibited this activity. This implies that the gene product is intracellularly localized in a way that allows access to the required components. If compared with V79 cells, the mutation rate for the hypoxanthine phosphoribosyltransferase (HPRT) locus in SD1 cells is markedly increased when exposed to aflatoxin B1, which is activated by this enzyme.
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单克隆抗体作为新城疫病毒 HN 糖蛋白的功能探针：血凝和神经氨酸酶位点的抗原分离。
DOI：
--
发表时间：
1984
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Iorio,RM;Bratt,MA
通讯作者：
Bratt,MA
影响因子：
3.7
作者：
A. Scheid;L. Caliguiri;R. Compans;P. Choppin
通讯作者：
P. Choppin
流感神经氨酸酶与单克隆抗体复合的电子和 X 射线衍射研究。
影响因子：
5.6
作者：
Tulloch,PA;Colman,PM;Davis,PC;Laver,WG;Webster,RG;Air,GM
通讯作者：
Air,GM
影响因子：
3.7
作者：
A. Scheid;P. Choppin
通讯作者：
P. Choppin
最近野外分离的仙台病毒具有温度敏感的 HN 糖蛋白。
影响因子：
3.7
作者：
Gorman,WL;Portner,A
通讯作者：
Portner,A