Formation and growth of Au nanoparticles inside live alfalfa plants
Formation and growth of Au nanoparticles inside live alfalfa plants
复制标题
DOI：
10.1021/nl015673
复制
发表时间：
2002-04-01
期刊：
影响因子：
10.8
通讯作者：
Yacaman, MJ
中科院分区：
文献类型：
--
作者：
Gardea-Torresdey, JL;Parsons, JG;Yacaman, MJ
在现代纳米技术中，最令人兴奋的领域之一是无机量子点与生物结构之间的相互作用。例如，被有机配体外壳包围的金簇可以共价连接到蛋白质或其他生物物质上，并可用于结构生物学的标记。在本报告中，我们展示了利用活植物制造纳米颗粒的可能性。紫花苜蓿植株生长在富含AUCl4的环境中，通过X射线吸收谱(XAS)和透射电子显微镜(TEM)证实了紫花苜蓿对Au的吸收。原子分辨率分析证实了Au纳米颗粒在植物内部的成核和生长，并且Au纳米颗粒处于结晶状态。图像还显示了晶体结构中的孪晶等缺陷，在某些情况下还发现了二十面体纳米粒子，X射线能谱仪研究证实了该纳米粒子是纯金。这是关于活植物形成金纳米颗粒的第一份报告，并开辟了制造纳米颗粒的新的令人兴奋的方法。它展示了如何在纳米生物技术这一新兴领域将材料科学和生物技术联系起来。
In modern nanotechnology one of the most exciting areas is the interaction between inorganic quantum dots and biological structures. For instance gold clusters surrounded by a shell of organic ligands covalently attach to proteins or other biological substances and can be used for labeling in structural biology. In the present report we show the possibility of using live plants for the fabrication of nanoparticles. Alfalfa plants were grown in an AUCl(4) rich environment, The absorption of Au metal by the plants was confirmed by X-ray absorption studies (XAS), and transmission electron microscopy (TEM). Atomic resolution analysis confirmed the nucleation and growth of Au nanoparticles inside the plant and that the Au nanoparticles are in a crystalline state. Images also showed defects such as twins in the crystal structure, and in some cases icosahedral nanoparticles were found, X-ray EDS studies corroborated that the nanoparticles are pure gold. This is the first report on the formation of gold nanoparticles by living plants and opens up new and exciting ways to fabricate nanoparticles. It shows how it is possible to link materials science and biotechnology in the new emerging field of nanobiotechnology.