Capping proteins regulate fungal development, DON-toxisome formation and virulence in Fusarium graminearum
Capping proteins regulate fungal development, DON-toxisome formation and virulence in Fusarium graminearum
复制标题
加帽蛋白调节禾谷镰刀菌的真菌发育、DON 毒素体形成和毒力
DOI：
10.1111/mpp.12887
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发表时间：
2020
影响因子：
4.9
通讯作者：
Ma Zhonghua
中科院分区：
文献类型：
--
作者：
Tang Guangfei;Chen Ahai;Dawood Dawood H.;Liang Jingting;Chen Yun;Ma Zhonghua
脱氧雪腐镰刀菌烯醇（DON）是由禾谷镰刀菌（Fusarium graminearum）产生的一种重要的镰刀菌毒素。DON在被称为毒素体的有组织内质网结构中合成。然而，毒素体形成的机制和毒素体的组分尚未完全了解。在先前的研究中，我们发现肌球蛋白I（FgMyo 1）-肌动蛋白细胞骨架参与了毒素体的形成。在目前的研究中，我们确定了两个新的组分的毒素体，肌动蛋白帽蛋白（CAP）FgCapA和FgCapB。这两个CAP在禾谷镰刀菌中形成异二聚体，并与FgMyo 1和Tri 1发生物理相互作用。缺失突变体Δ FgcapA和Δ FgcapA/B以及双缺失突变体ΔΔFgcapA/B显著抑制菌丝生长、无性繁殖和有性繁殖以及内吞作用。更重要的是，缺失突变体明显破坏了毒素体的形成和DON的产生，并减弱了植物的毒性。总的来说，这些结果表明，肌动蛋白CAPs与毒素体的形成，并有助于毒力和发展的F。禾谷早熟禾
Deoxynivalenol (DON) is an important trichothecene mycotoxin produced by the cereal pathogenFusarium graminearum. DON is synthesized in organized endoplasmic reticulum structures called toxisomes. However, the mechanism for toxisome formation and the components of toxisomes are not yet fully understood. In a previous study, we found that myosin I (FgMyo1)‐actin cytoskeleton participated in toxisome formation. In the current study, we identified two new components of toxisomes, the actin capping proteins (CAPs) FgCapA and FgCapB. These two CAPs form a heterodimer inF. graminearum, and physically interact with FgMyo1 and Tri1. The deletion mutants ΔFgcapAand ΔFgcapBand the double deletion mutant ΔΔFgcapA/Bdramatically reduced hyphal growth, asexual and sexual reproduction and endocytosis. More importantly, the deletion mutants markedly disrupted toxisome formation and DON production, and attenuated virulencein planta. Collectively, these results suggest that the actin CAPs are associated with toxisome formation and contribute to the virulence and development ofF. graminearum.