Modification by five antioxidants of 1,2-dimethylhydrazine-initiated colon carcinogenesis in F344 rats.
Modification by five antioxidants of 1,2-dimethylhydrazine-initiated colon carcinogenesis in F344 rats.
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五种抗氧化剂对 F344 大鼠中 1,2-二甲基肼引发的结肠癌发生的改变。
DOI：
10.1093/carcin/6.4.637
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发表时间：
1985
期刊：
影响因子：
4.7
通讯作者：
N. Ito
中科院分区：
文献类型：
--
作者：
T. Shirai;E. Ikawa;M. Hirose;W. Thamavit;N. Ito
研究了经1,2-二甲基肼（DMH）处理的雄性F344大鼠结肠肿瘤发生后给予抗氧化剂的影响。实验动物（20只/组）按20 mg/kg的剂量，每周1次，连续4周注射DMH。末次注射后1周，分别饲喂含5% l -抗坏血酸钠（SA）、0.5%丁基羟基茴香醚（BHA）、0.8%乙氧基醌（EQ）、1.0%没食子酸丙酯或0.5%丁基羟基甲苯（BHT）的日粮36周。对照组饲喂不含抗氧化剂的基础饲粮。第一次注射DMH后40周终止实验，所有肠道肿瘤组织学证实。SA显著增加了结肠腺瘤的发生率和每只大鼠（尤其是结肠远端）的肿瘤数量。虽然EQ和BHT对结肠肿瘤的数量没有影响，但EQ显著增加了每只大鼠远端结肠肿瘤的数量，而BHT则显著减少了肿瘤的数量。BHA和PG均未观察到对肿瘤发展的影响，因此，SA、EQ和BHT对肿瘤发展的影响是明显的，主要是在远端结肠。
The effects of antioxidants given in the post initiation phase of colon tumor development were investigated in male F344 rats treated with 1,2-dimethylhydrazine (DMH). Animals (20/group) were given s.c. injections of DMH at a dose of 20 mg/kg once a week for four consecutive weeks. One week after the last injection, rats were fed diet containing 5% sodium L-ascorbate (SA), 0.5% butylated hydroxyanisole (BHA), 0.8% ethoxyquin (EQ), 1.0% propyl gallate or 0.5% butylated hydroxytoluene (BHT) for 36 weeks. A control group was fed the basal diet not containing antioxidants. The experiment was terminated 40 weeks after the first injection of DMH and all intestinal tumors were confirmed histologically. SA significantly increased the incidence of adenomas and the number of tumors per rat of the colon (especially of the distal colon). Although EQ and BHT did not affect the number of rats with colon tumors, the number of tumors per rat occurring in the distal colon was significantly increased by EQ while being decreased by BHT. No modification of tumor development was observed with BHA or PG. Thus, modification of tumor development by SA, EQ and BHT was apparent, mainly in the distal colon.