Rho-Rho kinase pathway is involved in the regulation of myogenic tone and pump activity in isolated lymph vessels
Rho-Rho kinase pathway is involved in the regulation of myogenic tone and pump activity in isolated lymph vessels
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DOI：
10.1152/ajpheart.00763.2002
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发表时间：
2003-06-01
影响因子：
4.8
通讯作者：
Ohhashi, T
中科院分区：
文献类型：
--
作者：
Hosaka, K;Mizuno, R;Ohhashi, T
为探讨Rho-Rho激酶途径是否参与淋巴管机械活动的调节，观察了Y-27632和冈田酸对大鼠淋巴管淋巴泵活性及肌源性张力、压力张力和激动剂张力的影响。Y-27632引起淋巴泵活动停止，显著扩张。Y-27632还对去甲肾上腺素(NE)、U-46619或80 mMKCl预收缩的淋巴管产生剂量相关的扩张作用。冈田酸显著收缩淋巴管，降低淋巴泵活动频率。冈田酸还对NE或U-46619预先收缩的淋巴管产生与剂量相关的收缩。冈田酸可明显逆转Y-27632引起的淋巴泵活性降低。在Y-27632存在下，淋巴管的压力调节张力显著降低。另一方面，冈田酸显著增加了压力调节的音调。这些发现提示，淋巴管平滑肌中Rho激酶和肌球蛋白磷酸酶的活性可能参与淋巴泵活性的调节，也可能参与肌源性压力和激动剂诱导的张力的控制。
To evaluate whether or not Rho-Rho kinase pathway is involved in the regulation of mechanical activity of lymph vessels, effects of Y-27632 and okadaic acid on lymph pump activity and myogenic, pressure- and agonist-induced tone were examined in isolated rat lymph vessels. Y-27632 caused a significant dilation with a cessation of the lymph pump activity. Y-27632 also produced a dose-related dilation of the lymph vessels precontracted by norepinephrine (NE)-, U-46619- or 80 mM KCl. Okadaic acid significantly constricted the lymph vessels and reduced the frequency of the lymph pump activity. Okadaic acid also produced a dose-related constriction of the lymph vessels precontracted by NE or U-46619. The Y-27632-induced decrease of the frequency of lymph pump activity was significantly reversed by the pretreatment with okadaic acid. In the presence of Y-27632, the pressure- mediated tone of the lymph vessel was significantly decreased. On the other hand, okadaic acid significantly increased the pressure- mediated tone. These findings suggest that Rho kinase and myosin phosphatase activity in lymphatic smooth muscles may contribute to the regulation of lymph pump activity and may be also involved in the control of myogenic pressure- and agonist-induced tone.