Nonenzymatic oligomerization on templates containing phosphodiester-linked acyclic glycerol nucleic acid analogues
Nonenzymatic oligomerization on templates containing phosphodiester-linked acyclic glycerol nucleic acid analogues
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DOI：
10.1007/s002390010109
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发表时间：
2000-11-01
影响因子：
3.9
通讯作者：
Switzer, C
中科院分区：
文献类型：
--
作者：
Chaput, JC;Switzer, C
非酶低聚反应是研究益生元信息大分子复制的一种模型。为了探索无环寡核苷酸在这些反应中的适应性，我们合成了一系列DNA发夹，附加了含有无规甘油胞嘧啶残基的模板，发现无规甘油胞嘧啶单元阻碍而不是阻止鸟苷5'-磷-2-甲基咪唑（2-MeImpG）的模板定向寡聚。有证据表明，在给定模板位置的D和L甘油核苷构型都有助于产物的形成。还研究了含有分离甘油胞嘧啶残基的DNA双链的稳定性。双相热变性实验表明该化合物为无规糖基。dG碱基对的稳定性介于直流之间。dG对和dT。dG不匹配。
Nonenzymatic oligomerization reactions represent a model for studying the prebiotic replication of informational macromolecules. To explore the fitness of acyclic oligonucleotides in these reactions, we have synthesized a series of DNA hairpins appended with templates incorporating atactic glyceryl cytosine residues, Atactic glyceryl cytosine units are found to impede, but not to block, template-directed oligomerization of guanosine 5'-phosphoro-2-methylimidazole (2-MeImpG). Evidence suggests that both D and L glyceryl nucleoside configurations at a given template position contribute to product formation. The stability of DNA duplexes bearing isolated glyceryl cytosine residues has also been investigated. Duplex thermal denaturation experiments indicate that an atactic glyC.dG base-pair is intermediate in stability between a dC.dG pair and a dT.dG mismatch.