Cytochrome b barcoding, molecular systematics and geographic differentiation in rabbitfishes (Siganidae)
Cytochrome b barcoding, molecular systematics and geographic differentiation in rabbitfishes (Siganidae)
复制标题
DOI：
10.1016/j.crvi.2006.09.002
复制
发表时间：
2007-01-01
影响因子：
2
通讯作者：
Borsa, Philippe
中科院分区：
文献类型：
--
作者：
Lemer, Sarah;Aurelle, Didier;Borsa, Philippe
鱼类Siganus属(Siganidae)广泛分布于所有热带印度-太平洋的沿海生境，根据一般形态和颜色模式，迄今已确认28个命名种。根据细胞染色体b基因的部分核苷酸序列，建立了16个种的线粒体系统发育关系。给定命名种的个体单倍型通常聚集在长枝的末端，从而验证了当前的分类。然而，线虫单倍型形成了包括点滴虫在内的一个同源类群，而青海链霉单倍型明显分裂成两个类群，需要进一步研究。虽然它们之间有14.5%-16.3%的核苷酸距离，但从它们在形态上的亲缘关系来看，它们形成了一个单系群。在印度-西太平洋不同地点采集的8种物种中，银鱼和刺鱼的地理分化程度最低，这一结果与它们延长的远洋幼虫阶段有很好的相关性。在所有其他六种被测试的物种(斑点斑潜蝇、青海斑潜蝇、斑点斑潜蝇、斑点斑潜蝇)中，固定指数估计值都很高。这里选择的细胞色素b基因片段被证明是一种有用的条形码。
The fish genus Siganus (Siganidae) is widely distributed in the coastal habitats of all the tropical Indo-Pacific, with 28 nominal species recognized so far, based on general morphology and coloration patterns. A mitochondrial phylogeny of 16 Siganidae species, based on the partial nucleotide sequences of the cytochome b gene, was produced. Individual haplotypes of given nominal species generally clustered at the extremity of long branches, thus validating the current taxonomy. However, S. lineatus haplotypes formed a paraphyletic group including S. guttatus, while S. fuscescens haplotypes were apparently splitted in two groups, calling for further investigation. S. woodlandi and S. argenteus formed a monophyletic group, as expected from their close morphological relatedness, although they were separated by a substantial, 14.5-16.3% nucleotide distance. Among eight species sampled from different locations across the Indo-West Pacific, S. argenteus and S. spinus showed the lowest degree of geographic differentiation, a result that correlated well with their extended pelagic larval stage. Fixation index estimates were high in all six other species tested (S. doliatus, S. fuscescens, S. lineatus, S. puellus, S. punctatus, S. vulpinus). The cytochrome b gene fragment chosen here proved useful as a barcode in Siganidae.