ER function in the adult male rat: short- and long-term effects of the antiestrogen ICI 182,780 on the testis and efferent ductules, without changes in testosterone.
ER function in the adult male rat: short- and long-term effects of the antiestrogen ICI 182,780 on the testis and efferent ductules, without changes in testosterone.
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DOI：
10.1210/endo.143.6.8873
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发表时间：
2002-06
期刊：
影响因子：
4.8
通讯作者：
Cleida A Oliveira;Qing Zhou;K. Carnes;Rong Nie;D. Kuehl;G. L. Jackson;L. R. França;M. Nakai;R. Hess
中科院分区：
文献类型：
--
作者：
Cleida A Oliveira;Qing Zhou;K. Carnes;Rong Nie;D. Kuehl;G. L. Jackson;L. R. França;M. Nakai;R. Hess
用抗雌激素ICI 182780治疗30 d的雄性大鼠（3-150 d），以确定导致睾丸萎缩和不育的事件顺序。血浆睾酮和LH浓度不变。ICI 182780早在治疗后3 d诱导传出小管扩张，随着时间的推移，扩张增加，导致小管直径总体增加200%。观察到小管上皮高度逐渐降低；微绒毛高度在73 d时呈上升趋势，但随后又呈下降趋势。从第15天到第100天，非纤毛细胞中溶酶体的短暂增加。睾丸重量增加45 d，精管扩张52 d。这些对睾丸的影响一直持续到100 d，但在150 d时体重下降并出现严重萎缩。这些睾丸效应可能是由于在传出小管中重吸收抑制后液体的积累，类似于er - α敲除小鼠。与此结论一致的是，Na+-H+交换器-3 mRNA和蛋白质的减少，这与先前的研究结果一致，表明ER是Na+-H+交换器-3表达和最终在输出小管中液体重吸收所必需的。
Male rats, 30 d old, were treated with the antiestrogen ICI 182,780 (3-150 d) to determine sequences of events leading to testicular atrophy and infertility. Plasma testosterone and LH concentrations were unchanged. ICI 182,780 induced dilation of efferent ductules as early as 3 d post treatment, and the dilation increased over time, resulting in an overall increase of 200% in tubule diameter. A gradual reduction in height of the ductule epithelium was observed; however, the microvilli height increased up to d 73 but subsequently decreased. A transient increase in lysosomes in nonciliated cells was seen from d 15 to d 100. Testicular weight increased by d 45 and seminiferous tubules were dilated by d 52. These effects on testes persisted until d 100, but on d 150 the weight decreased and severe atrophy was observed. These testicular effects were probably owing to accumulation of fluid following inhibition of reabsorption in the efferent ductules, similar to the ER-alpha knockout mouse. In agreement with this conclusion, there was a decrease in Na+-H+ exchanger-3 mRNA and protein, which is consistent with previous studies showing that ER is required for expression of Na+-H+ exchanger-3 and ultimately fluid reabsorption in the efferent ductules.