Molecular analysis of gene expression in the developing pontocerebellar projection system
Molecular analysis of gene expression in the developing pontocerebellar projection system
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DOI：
10.1016/s0896-6273(02)01016-4
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发表时间：
2002-10-24
期刊：
影响因子：
16.2
通讯作者：
Scheiffele, P
中科院分区：
文献类型：
--
作者：
Díaz, E;Ge, YC;Scheiffele, P
作为了解神经元分化的分子机制的一种方法，我们利用DNA微阵列来阐明脑桥小脑发育过程中基因表达的全球模式。通过这种分析，我们确定了神经元前体细胞特异性的基因组，与轴突生长相关，并在与突触靶细胞接触时进行调节。在小脑，我们确定了一个阶段的颗粒细胞分化，是独立的相互作用与其他小脑细胞类型。脑桥基因表达的分析表明，不同的程序的基因表达，与轴突生长和突触的形成，可以解耦，并可能受到不同的细胞在小脑的目标环境。我们的方法提供了深入了解潜在的特定细胞类型的分化在脑桥小脑投射系统的遗传程序。
As an approach toward understanding the molecular mechanisms of neuronal differentiation, we utilized DNA microarrays to elucidate global patterns of gene expression during pontocerebellar development. Through this analysis, we identified groups of genes specific to neuronal precursor cells, associated with axon outgrowth, and regulated in response to contact with synaptic target cells. In the cerebellum, we identified a phase of granule cell differentiation that is independent of interactions with other cerebellar cell types. Analysis of pontine gene expression revealed that distinct programs of gene expression, correlated with axon outgrowth and synapse formation, can be decoupled and are likely influenced by different cells in the cerebellar target environment. Our approach provides insight into the genetic programs underlying the differentiation of specific cell types in the pontocerebellar projection system.