Direct evidence for antifreeze glycoprotein adsorption onto an ice surface.
Direct evidence for antifreeze glycoprotein adsorption onto an ice surface.
复制标题
抗冻糖蛋白吸附到冰表面的直接证据。
DOI：
10.1002/bip.360240713
复制
发表时间：
1985
期刊：
影响因子：
2.9
通讯作者：
Feeney,RE
中科院分区：
文献类型：
--
作者：
Brown,RA;Yeh,Y;Burcham,TS;Feeney,RE
抗冻糖蛋白（AFGP）的水溶液表现出冻结和融化温度之间的滞后。最近对这种蛋白质系统的功能机制的几项研究表明，一个可能的模型是防冻剂分子被吸附到晶体表面上。然而，吸附AFGP的存在的直接证据已经回避了以前的研究人员。在本研究中，在与纯冰单晶接触的活性AFGP溶液的存在下观察到增强的表面二次谐波产生（SSHG）。SSHG的增强是活性AFGP分子吸附到冰晶表面的积极迹象。
Aqueous solutions of antifreeze glycoproteins (AFGP) exhibit hysteresis between the freezing and melting temperatures. Several recent studies on the mechanism of function of this protein system suggest that a likely model is for the antifreeze molecules to be adsorbed onto the surface of the crystals. However, direct proof of the presence of adsorbed AFGP has eluded previous researchers. In the present study, enhanced surface second‐harmonic generation (SSHG) was observed in the presence of an active AFGP solution in contact with a pure single crystal of ice. The enhancement of SSHG is a positive indication that active AFGP molecules adsorb to the surface of ice crystals.