GnRH agonist Buserelin affects colony-forming efficiency of HHUA and Jurkat cells.
GnRH agonist Buserelin affects colony-forming efficiency of HHUA and Jurkat cells.
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GnRH 激动剂 Buserelin 影响 HHUA 和 Jurkat 细胞的集落形成效率。
DOI：
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发表时间：
2001
期刊：
影响因子：
--
通讯作者：
M. Park
中科院分区：
文献类型：
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作者：
M. Enomoto;T. Mori;M. Park
在这项研究中，在低和高细胞密度下检查细胞增殖，使用HHUA和Jurkat细胞系作为GnRH激动剂Buserelin的抗增殖和增殖促进作用的实验模型。为了在低细胞密度下有效评价布舍瑞林活性，采用集落形成效率测定。在低细胞密度下，布舍瑞林以剂量依赖性方式显著影响集落形成效率;然而，布舍瑞林在高细胞密度下无影响。HHUA细胞的条件培养基抑制布舍瑞林的作用，而Jurkat细胞的条件培养基则模拟布舍瑞林的作用，这些结果表明，每个细胞系都分泌一些调节细胞增殖的物质，这些物质也能改变布舍瑞林的作用。集落形成效率的测量是消除自分泌和/或旁分泌效应的非常有效的方法，并且是测量GnRH活性的高度灵敏的方法。
In this study, cell proliferation was examined at low and high cell densities, using HHUA and Jurkat cell lines as experimental models for the antiproliferative and proliferation-enhancing effects of GnRH agonist, Buserelin, respectively. For efficient evaluation of Buserelin activity at low cell density, the colony-forming efficiency assay was adopted. Buserelin markedly affected colony-forming efficiency in a dose-dependent manner at low cell density; however, Buserelin had no effect at high cell density. The conditioned medium of HHUA cells inhibited the Buserelin action, whereas that of Jurkat cells mimicked it. These results suggest that each cell line secretes some substances which regulate cell proliferation, and that these substances can also change the effects of Buserelin. The measurement of colony-forming efficiency is a very effective way of eliminating autocrine and/or paracrine effects, and is a highly sensitive method for measuring GnRH activity.
影响因子：
5.8
作者：
Papadopoulos,V;Dharmarajan,AM;Li,H;Culty,M;Lemay,M;Sridaran,R
通讯作者：
Sridaran,R
用黄体生成素释放激素和生长抑素类似物治疗后仓鼠胰腺癌的程序性细胞死亡（细胞凋亡）。
DOI：
10.1073/pnas.86.5.1643
发表时间：
1989
影响因子：
11.1
作者：
Szende,B;Zalatnai,A;Schally,AV
通讯作者：
Schally,AV