Cytochrome c1-like is required for mitochondrial morphogenesis and individualization during spermatogenesis in Drosophila melanogaster
Cytochrome c1-like is required for mitochondrial morphogenesis and individualization during spermatogenesis in Drosophila melanogaster
复制标题
细胞色素 c1 样是果蝇精子发生过程中线粒体形态发生和个体化所必需的
DOI：
10.1242/jeb.245277
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发表时间：
2023
影响因子：
2.8
通讯作者：
Yu-Feng Wang
中科院分区：
文献类型：
--
作者：
Meng-Yan Chen;Xin Duan;Qian Wang;Mao-Jiu Ran;Hui Ai;Ya Zheng;Yu-Feng Wang
果蝇睾丸是研究雄性生殖细胞增殖、初级精母细胞减数分裂、线粒体形态发生和精子细胞个体化等从生殖细胞到活动精子过程的良好系统。我们先前已经证明，眼球(ocnus，ocn)在男性生殖细胞发育中起着重要的作用。在ocn基因敲除后表达水平发生改变的基因和蛋白中，细胞色素C1样基因(cyt-c1L)显著下调。在这里，我们发现Cyt-c1L在黑腹水母的睾丸中高表达。果蝇早期生殖细胞Cyt-c1L基因敲除或突变可导致雄性不育。免疫荧光染色显示，Cyt-c1L基因敲除的睾丸在早期精子发生中没有缺陷，但在后期，与对照睾丸中不同位置的F-肌动蛋白锥体组成的个性化复合体(ICs)不同，Cyt-c1L基因敲除睾丸中没有观察到肌动蛋白锥体或ICs。此外，Cyt-c1L基因敲除睾丸的精囊内未见成熟精子，而对照精囊内充满了针状核的成熟精子。Cyt-c1L基因敲除还导致精子细胞伸长过程中线粒体形态发生异常。Cyt-c1L基因敲除的苍蝇睾丸底部聚集过多的凋亡信号。这些结果表明，Cyt-c1L可能通过影响线粒体的形态发生和精子的个体化而在精子发生中发挥重要作用。
Drosophila testis is an excellent system for studying the process from germ stem cells to motile sperm, including the proliferation of male germ cells, meiosis of primary spermatocyte, mitochondrial morphogenesis, and spermatid individualization. We previously demonstrated that the ocnus (ocn) plays an essential role in male germ cell development. Among those genes and proteins whose expression levels were changed due to ocn knockdown, the cytochrome c1-like (cyt-c1L) was downregulated significantly. Here, we showed that cyt-c1L was highly expressed in the testis of D. melanogaster. Knockdown or mutation of cyt-c1L in early germ cells of flies resulted in male sterility. Immunofluorescence staining exhibited that cyt-c1L knockdown testis had no defects in early spermatogenesis; however, in late stages, contrast to many individualization complexes (ICs) composed by F-actin cones appeared at different positions in control testes, no actin cones or ICs were observed in cyt-c1L knockdown testes. Furthermore, no mature sperms were found in the seminal vesicle of cyt-c1L knockdown testes whereas the control seminal vesicle was full of mature sperms with needle-like nuclei. cyt-c1L knockdown also caused abnormal mitochondrial morphogenesis during spermatid elongation. Excessive apoptotic signals were accumulated in the base of cyt-c1L knockdown fly testes. These results suggest that cyt-c1L may play an important role in spermatogenesis by affecting the mitochondrial morphogenesis and individualization of sperms in D. melanogaster.