Delayed neuronal cell death and microglial cell reactivity in the CA1 region of the rat hippocampus in the cardiac arrest model
Delayed neuronal cell death and microglial cell reactivity in the CA1 region of the rat hippocampus in the cardiac arrest model
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DOI：
10.1007/bf01557785
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发表时间：
1998-01-01
期刊：
影响因子：
--
通讯作者：
Matsumoto, Kiyoshi
中科院分区：
文献类型：
--
作者：
Dohi, Kenji;Shioda, Seiji;Matsumoto, Kiyoshi
采用原位缺口末端标记（TUNEL）方法以及光镜和电镜研究了大鼠心脏骤停模型海马CA1区的形态变化。 TUNEL阳性锥体细胞在第1天首次出现，数量随时间增加，并在再循环后7天达到峰值。在超微结构水平上，在 CA1 区域观察到细胞收缩、核碎裂和锥体细胞自噬液泡数量增加。短暂的缺血会激活 CA1 区域的小胶质细胞，并且这些细胞的数量随着时间的推移而增加。小胶质细胞粘附在退化的锥体细胞上并选择性地吞噬凋亡的神经元。
Morphological changes in the CA1 region of the hippocampus in the rat cardiac arrest model were studied with the in situ nick-end labeling (TUNEL) method and light and electron microscopy. The TUNEL-positive pyramidal cells first appeared on day 1, increased in number with time, and reached a peak at 7 days after recirculation. At the ultrastructural level, cell shrinkage, nuclear fragmentation, and an increased number of atuophagic vacuoles of the pyramidal cells were observed in the CA1 region. The brief ischemia activates the microglial cells in the CA1 region, and these cells were found to increase in number with time. The microglial cells were seen to adhere to degenerating pyramidal cells and to phagocytose the apoptotic neurons selectively.