Mobilization of a transposon in the rice genome
Mobilization of a transposon in the rice genome
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DOI：
10.1038/nature01219
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发表时间：
2003-01-09
期刊：
影响因子：
64.8
通讯作者：
Tanisaka, T
中科院分区：
文献类型：
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作者：
Nakazaki, T;Okumoto, Y;Tanisaka, T
水稻（Oryza sativa L.）是世界范围内的重要作物，并且随着序列草案的可用性（1，2），是用于分析禾本科植物基因组结构的有用模型（3），（4）。为了利用基因工程技术进行水稻高效育种，鉴定水稻中具有重要经济价值的基因是非常重要的。在完整植物中使用移动的转座子作为基因标签是功能分析的有力工具，因为转座子插入通常会使基因突变（5）。在这里，我们通过分析颖壳（包围并决定谷粒形状的种子结构）的细长突变的突变性来鉴定一种名为微型Ping（mPing）的活性水稻转座子。mPing转座子插入到细长颖（slg）突变体等位基因中，但不插入到野生型等位基因中。搜索O。在水稻品种日本晴基因组中，共鉴定出34个与mPing具有高度相似性的序列，表明mPing是一个转座子家族。从slg植物切除mPing导致回复到野生型表型。转座子mPing在完整水稻植株中的移动性为水稻基因的功能分析提供了一个有用的替代工具。
Rice (Oryza sativa L.) is an important crop worldwide and, with the availability of the draft sequence(1,2), a useful model for analysing the genome structure of grasses(3),(4). To practice efficient rice breeding through genetic engineering techniques, it is important to identify the economically important genes in this crop. The use of mobile transposons as gene tags in intact plants is a powerful tool for functional analysis because transposon insertions often inactivate genes(5). Here we identify an active rice transposon named miniature Ping (mPing) through analysis of the mutability of a slender mutation of the glume(6)-the seed structure that encloses and determines the shape of the grain. The mPing transposon is inserted in the slender glume (slg) mutant allele but not in the wild-type allele. Search of the O. sativa variety Nipponbare genome identified 34 sequences with high nucleotide similarity to mPing, indicating that mPing constitutes a family of transposon elements. Excision of mPing from slg plants results in reversion to a wild-type phenotype. The mobility of the transposon mPing in intact rice plants represents a useful alternative tool for the functional analysis of rice genes.