Dynamic co-expression network analysis of lncRNAs and mRNAs associated with venous congestion.
Dynamic co-expression network analysis of lncRNAs and mRNAs associated with venous congestion.
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DOI：
10.3892/mmr.2016.5480
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发表时间：
2016-09
影响因子：
3.4
通讯作者：
Wu L
中科院分区：
文献类型：
--
作者：
Li J;Xu Y;Xu J;Wang J;Wu L
静脉充血和容量超负荷在心肾综合征中是重要的，其中涉及多种调节因子，包括长链非编码RNA（lncRNA）。为了研究lncRNA在静脉充血发生中的作用，首先对与外周静脉充血相关的Affyssin微阵列进行注释，然后构建lncRNA-mRNA双向动态共表达网络，网络节点分别表示lncRNA或mRNA。当lncRNA或mRNA动态共表达时，节点连接。在对该网络进行功能分析后，确定了几种动态替代途径，包括静脉充血发展过程中的钙信号通路。此外，某些lncRNA（LINC 00523，LINC 01210和RP 11 -435O5.5）被鉴定为可能动态调节钙信号通路中的某些蛋白质，包括质膜钙ATP酶（PMCA）和G蛋白偶联受体（GPCR）。特别地，LINC 00523从与PMCA共表达到GPCR的动态调节转换可能涉及稳态细胞内钙的损伤。简而言之，目前的研究证明了静脉充血期间lncRNA功能的潜在新机制。
Venous congestion and volume overload are important in cardiorenal syndromes, in which multiple regulated factors are involved, including long non-coding RNAs (lncRNAs). To investigate the underlying role of lncRNAs in regulating the development of venous congestion, an Affymetrix microarray associated with peripheral venous congestion was annotated, then a bipartite dynamic lncRNA-mRNA co-expression network was constructed in which nodes indicated lncRNAs or mRNAs. The nodes were connected when the lncRNAs or mRNAs were dynamically co-expressed. Following functional analysis of this network, several dynamic alternative pathways were identified, including the calcium signaling pathway during venous congestion development. Additionally, certain lncRNAs (LINC00523, LINC01210 and RP11-435O5.5) were identified that may potentially dynamically regulate certain proteins, including plasma membrane calcium ATPase (PMCA) and G protein-coupled receptor (GPCR), in the calcium signaling pathway. Particularly, the dynamically regulated switch of LINC00523 from co-expression with PMCA to GPCR may be involved in damage to steady state intracellular calcium. In brief, the current study demonstrated a potential novel mechanism of lncRNA function during venous congestion.