Sequential structure of neocortical spontaneous activity in vivo
Sequential structure of neocortical spontaneous activity in vivo
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DOI：
10.1073/pnas.0605643104
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发表时间：
2007-01-02
影响因子：
11.1
通讯作者：
Harris, Kenneth D.
中科院分区：
文献类型：
--
作者：
Luczak, Artur;Bartho, Peter;Harris, Kenneth D.
即使在没有感觉刺激的情况下，新皮层也显示出复杂的自发活动模式，通常由交替的“向下”状态的广义神经沉默和“向上”状态的大规模持续网络活动组成。为了研究这种自发活动如何在体内通过神经元组件传播，我们同时记录了麻醉和清醒大鼠第V层中50-200个皮层神经元的群体。每个神经元在UP状态期间显示出几乎独特的尖峰模式，在假定的锥体细胞和中间神经元之间观察到多样性，反映了通过局部皮层网络激活的复杂但刻板组织的顺序传播。尖峰时间是最精确的第一次约100毫秒后UP状态发作，并衰减UP状态的进展。UP状态的一个子集作为行波传播，但是波在任一方向上通过给定点引发了类似的局部序列，这表明局部网络是顺序发射模式的基底。对重复基序的搜索表明，它们的发生和结构是可预测的，从神经元的个体潜伏期到UP状态开始。我们认为，这些刻板的模式产生的内在细胞电导和局部电路特性的相互作用。
Even in the absence of sensory stimulation, the neocortex shows complex spontaneous activity patterns, often consisting of alternating "DOWN" states of generalized neural silence and "UP" states of massive, persistent network activity. To investigate how this spontaneous activity propagates through neuronal assemblies in vivo, we simultaneously recorded populations of 50-200 cortical neurons in layer V of anesthetized and awake rats. Each neuron displayed a virtually unique spike pattern during UP states, with diversity seen amongst both putative pyramidal cells and inter-neurons, reflecting a complex but stereotypically organized sequential spread of activation through local cortical networks. Spike timing was most precise during the first approximate to 100 ms after UP state onset, and decayed as UP states progressed. A subset of UP states propagated as traveling waves, but waves passing a given point in either direction initiated similar local sequences, suggesting local networks as the substrate of sequential firing patterns. A search for repeating motifs indicated that their occurrence and structure was predictable from neurons' individual latencies to UP state onset. We suggest that these stereotyped patterns arise from the interplay of intrinsic cellular conductances and local circuit properties.