A feedback regulation of FgHtf1-FgCon7 loop in conidiogenesis and development of Fusarium graminearum
A feedback regulation of FgHtf1-FgCon7 loop in conidiogenesis and development of Fusarium graminearum
复制标题
DOI：
10.1016/j.ijbiomac.2024.129841
复制
发表时间：
2024-02-06
影响因子：
8.2
通讯作者：
Zheng，Huawei
中科院分区：
文献类型：
--
作者：
Chen，Shuang;Li，Pengfang;Zheng，Huawei
转录因子FgHtf1在稻瘟病菌孢子形成中起着重要的作用，它正调控着产孢相关基因FgHtf1的表达。然而，其功能背后的调控机制尚不清楚。本研究旨在揭示FgHtf1与FgCon7的作用机制。我们证明fgcon7是FgHtf1的靶基因。有趣的是，FgCon7还结合启动子区域offghtf1负向调控其表达，从而形成负反馈回路。我们证明FgHtf1和FgCon7在真菌发育中具有功能冗余。FgCon7定位于细胞核并具有转录激活活性。缺失offgcon7显著减少分生孢子的产生。ChIP-Seq中4444个基因受FgCon7调控，RNA-Seq显示4430个差异表达基因FgCon7缺失突变，CCAAT作为FgCon7与靶基因的一致结合基序。FgCon7直接结合gmsn2、FgABAA、fgvea和fgsmt3基因的启动子区域，调控其表达。这些基因在分生过程中起重要作用。据我们所知，这是第一次研究揭示了fgcon7和fghtf1相互调节作用，形成负反馈回路，以及该回路如何调节谷草菌的孢子形成。
The transcription factor FgHtf1 is important for conidiogenesis inFusarium graminearumand it positively regulates the expression of the sporulation-related geneFgCON7. However, the regulatory mechanism underlying its functions is still unclear. The present study intends to uncover the functional mechanism of FgHtf1 in relation to FgCon7 inF. graminearum. We demonstrated thatFgCON7serves as a target gene for FgHtf1. Interestingly, FgCon7 also binds the promoter region ofFgHTF1to negatively regulate its expression, thus forming a negative-feedback loop. We demonstrated that FgHtf1 and FgCon7 have functional redundancy in fungal development. FgCon7 localizes in the nucleus and has transcriptional activation activity. Deletion ofFgCON7significantly reduces conidia production. 4444 genes were regulated by FgCon7 in ChIP-Seq, and RNA-Seq revealed 4430 differentially expressed genes inFgCON7deletion mutant, with CCAAT serving as a consensus binding motif of FgCon7 to the target genes. FgCon7 directly binds the promoter regions ofFgMSN2,FgABAA,FgVEAandFgSMT3genes and regulates their expression. These genes were found to be important for conidiogenesis. To our knowledge, this is the first study that unveiled the mutual regulatory functions ofFgCON7andFgHTF1to form a negative-feedback loop, and how the loop mediates sporulation inF. graminearum.