PROTEOLYTIC ACTIVITY IN EXTRACTS OF INVASIVE CERVICAL-CARCINOMA AND PRECURSOR LESIONS
PROTEOLYTIC ACTIVITY IN EXTRACTS OF INVASIVE CERVICAL-CARCINOMA AND PRECURSOR LESIONS
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DOI：
10.1016/0753-3322(96)82648-5
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发表时间：
1995-01-01
影响因子：
7.5
通讯作者：
PEREZMONTFORT, R
中科院分区：
文献类型：
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作者：
DANERINAVARRO, A;DELTOROARREOLA, S;PEREZMONTFORT, R
在这份初步报告中，我们发现，蛋白水解活性的提取物从85例正常宫颈，低，高级别鳞状上皮内病变和浸润性癌患者的宫颈样本，增加根据自然历史的宫颈癌时，测量三种不同的基板。四个不同的催化类内肽酶的抑制剂测定表明，在所有组的提取物中的主要催化类是金属蛋白酶。底物凝胶电泳显示，浸润性癌提取物具有两条蛋白水解活性条带（M（r）为72和52 kDa），这在正常组织或具有前驱病变的活检组织中不存在。这些条带的免疫学和分子生物学特征可能提供与宫颈癌生物学和临床应用相关的信息。
In this preliminary report, we showed that proteolytic activity of extracts from 85 cervical samples of patients with normal cervix, low and high-grade squamous intra-epithelial lesions and invasive carcinoma, increased according to the natural history of the cervical cancer when measured with three different substrates. Inhibitor assays for four different catalytic classes of endopeptidases indicated that the predominant catalytic class in extracts of all groups was that of metalloproteinases. Substrate gel electrophoresis revealed that invasive carcinoma extracts had two bands with proteolytic activity (with M(r) of 72 and 52 kDa) which were not present in normal tissue or biopsies with precursor lesions. Immunological and molecular characterization of these bands may provide information relevant to cervical cancer biology and clinical applications.