Live-Cell Imaging of Cytoskeletal Responses and Trafficking During Fungal Elicitation.
Live-Cell Imaging of Cytoskeletal Responses and Trafficking During Fungal Elicitation.
复制标题
真菌诱导过程中细胞骨架反应和运输的活细胞成像。
DOI：
10.1007/978-1-0716-2867-6_22
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Connerton AJ
中科院分区：
文献类型：
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作者：
Connerton AJ
了解植物防御反应的驱动机制有望通过农用化学品和有针对性的遗传改良提供新的手段来加强植物防御。当阐明特定毒力机制的作用时，量化植物病原体对亚细胞动力学的影响的能力是特别重要的，所述毒力机制对感染成功做出贡献，但不单独改变疾病结果。获取这些数据需要研究人员在观察之前成功处理植物和微生物，并了解在这些条件下获取图像的挑战。在这一章中，我们描述了一个协议，以支持周围的真菌相互作用，特别是白粉病Blumeria graminisf.sp.hordeion的表面ofArabidopsis thaliana网站的细胞骨架动力学观察。此外，我们还描述了一个程序，暴露黄化（黑暗生长）下胚轴的分子模式，以激活防御反应的情况下，植物病原体的目的是观察本地化的肌动蛋白依赖的贩运。
Understanding the mechanisms driving plant defense responses holds the promise to provide new means to reinforce plant defense both through agrochemicals and targeted genetic improvement. The capability to quantify impacts of phytopathogens on subcellular dynamics is particularly important when elucidating the role of specific virulence mechanisms that make contributions toward infection success but do not individually alter disease outcome. Acquiring these data requires an investigator to achieve the successful handling of both plant and microbe prior to observation and an appreciation of the challenges in acquiring images under these conditions. In this chapter we describe a protocol to support the observation of cytoskeletal dynamics surrounding sites of fungal interaction, specifically the powdery mildewBlumeria graminisf.sp.hordeion the surface ofArabidopsis thaliana. Furthermore, we also describe a procedure to expose etiolated (dark-grown) hypocotyls to a molecular pattern to activate defense responses in the absence of a phytopathogen with the aim of observing localized actin-dependent trafficking.