Microsatellites in the silkworm, Bombyx mori:: Abundance, polymorphism, and strain characterization
Microsatellites in the silkworm, Bombyx mori:: Abundance, polymorphism, and strain characterization
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DOI：
10.1139/gen-42-6-1057
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发表时间：
1999-12-01
期刊：
影响因子：
3.1
通讯作者：
Nagaraju, J
中科院分区：
文献类型：
--
作者：
Reddy, KD;Abraham, EG;Nagaraju, J
我们已经从家蚕基因组中分离和鉴定了微卫星（简单重复序列（SSR）位点）。通过常规杂交方法筛选部分基因组文库，分离出28个含有微卫星的克隆。家蚕基因组中（CA）（n）重复序列的丰度与其他生物如蜜蜂、猪和人类中报道的相似，但与熊蜂和蜜蜂基因组相比，（CT）（n）重复序列基序不太常见。用15个微卫星位点对13个家蚕品种进行了分析，结果表明，等位基因数为3 ~ 17个，杂合度为0.66 ~ 0.90。沿着品系特异的微卫星标记，还鉴定了滞育和非滞育品系特异的等位基因。在本研究中，重复序列长度与多态性程度没有任何关系。F1代的微卫星标记表现为共显性遗传。提供了标记每个基因座的引物序列的列表。微卫星标记的应用有望提高家蚕基因组分析的能力和分辨率。
We have isolated and characterized microsatellites (simple sequence repeat (SSR) loci) from the silkworm genome. The screening of a partial genomic library by the conventional hybridization method led to the isolation of 28 microsatellites harbouring clones. The abundance of (CA)(n) repeats in the silkworm genome was akin to those reported in the other organisms such as honey bee, pig, and human, but the (CT)(n) repeat motif is less common compared to bumble bee and honey bee genomes. Detailed analysis of 13 diverse silkworm strains with a representative of 15 microsatellite loci revealed a number of alleles ranging from 3 to 17 with heterozygosity values of 0.66-0.90. Along with strain-specific microsatellite markers, diapause and non-diapause strain-specific alleles were also identified. The repeat length did not show any relationship with the degree of polymorphism in the present study. The co-dominant inheritance of microsatellite markers was demonstrated in F-1 offspring. A list of primer sequences that tag each locus is provided. The availability of microsatellite markers can be expected to enhance the power and resolution of genome analysis in silkworm.