Measurement of liver T1 and T2 relaxation times in an experimental mouse model of liver fibrosis
Measurement of liver T1 and T2 relaxation times in an experimental mouse model of liver fibrosis
复制标题
DOI：
10.1002/jmri.23606
复制
发表时间：
2012-07-01
影响因子：
4.4
通讯作者：
Wu, Ed X.
中科院分区：
文献类型：
--
作者：
Chow, April M.;Gao, Darwin S.;Wu, Ed X.
目的：用定量磁共振成像(MRI)研究实验性肝纤维化小鼠肝脏松弛时间的变化。定量MRI是一种潜在的强有力的方法来表征肝纤维化。然而，松弛时间与纤维化分期之间的相关性一直存在争议。材料和方法：成年雄性C57BL/6N小鼠(2225g；n=12)采用四氯化碳(CCl4)重复给药诱导肝纤维化模型。在CCl4损伤前、损伤后2周、4周、6周和8周对动物进行一系列不同TRs的自旋回波(SE)成像和MultiECHO SE成像序列的检查。测量肝脏T1、T2值。组织学采用苏木精-伊红染色和Masson‘s三色染色。结果：与染毒前相比，染毒后2、4、6、8周肝组织T1均显著升高(P<0.001)。同时，肝脏T2在染毒后2、4、6、8周显著高于染毒前(P<；0.001)。肝组织学检查显示肝组织胶原沉积、水肿、肝组织炎性细胞渗出。结论：纵向和横向弛豫时间均可作为肝纤维化的可靠指标。随着单次屏气磁共振弛豫序列的出现，可以常规可靠地在腹部器官进行弛豫时间的定量标测，因此在早期发现肝纤维化和监测其进展方面可能是有价值的和可靠的。J·马根。雷森。《成像》2012年；36：152158。(C)2012年威利期刊公司。
Purpose: To characterize changes in relaxation times of liver using quantitative magnetic resonance imaging (MRI) in an experimental mouse model of liver fibrosis. Quantitative MRI is a potentially robust method to characterize liver fibrosis. However, correlation between relaxation times and fibrosis stage has been controversial. Materials and Methods: Liver fibrosis was induced in male adult C57BL/6N mice (2225 g; n = 12) by repetitive dosing of carbon tetrachloride (CCl4). The animals were examined with a series of spin-echo (SE) images with varying TRs and multiecho SE imaging sequence at 7 T before and 2, 4, 6, and 8 weeks after CCl4 insult. Hepatic T1 and T2 values were measured. Histology was performed with hematoxylin-eosin staining and Masson's trichrome staining. Results: Significant increase (P < 0.001) in hepatic T1 was found at 2, 4, 6, and 8 weeks following CCl4 insult as compared with that before insult. Meanwhile, hepatic T2 at 2, 4, 6, and 8 weeks after CCl4 insult was significantly higher (P < 0.001) than that before the insult. Liver histology showed collagen deposition, edema, and infiltration of inflammatory cells in livers with CCl4 insult. Conclusion: Both longitudinal and transverse relaxation times may serve as robust markers for liver fibrosis. With the advent of single breath-hold sequences for MR relaxometry, quantitative mapping of relaxation times can be routinely and reliably performed in abdominal organs and hence may be valuable and robust in detecting liver fibrosis at early phase and monitoring its progression. J. Magn. Reson. Imaging 2012;36:152158. (c) 2012 Wiley Periodicals, Inc.