Cationic poly(lactide-co-glycolide) nanoparticles as efficient in vivo gene transfection agents
Cationic poly(lactide-co-glycolide) nanoparticles as efficient in vivo gene transfection agents
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DOI：
10.1166/jnn.2004.130
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发表时间：
2004-11-01
影响因子：
--
通讯作者：
Lehr, CM
中科院分区：
文献类型：
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作者：
Kumar, MNVR;Mohapatra, SS;Lehr, CM
聚乳酸-羟基乙酸酯（PLGA）是一种具有生物相容性和可生物降解的聚乳酸和聚乳酸共聚物，因其具有传递基因的能力而得到认可。然而，PLGA纳米颗粒的基因传递受到其负电荷和通过粘膜屏障的运输能力的限制。在本研究中，利用阳离子壳聚糖对PLGA纳米颗粒进行表面修饰，以提高其基因传递能力。以pva -壳聚糖（PLGA1）或pva -壳聚糖- peg （PLGA2）共混物为稳定剂，采用乳化-扩散-蒸发法制备了PLGA纳米颗粒。该方法重现性好，制备的纳米颗粒具有流体动力学直径
Poly(lactide-co-glycolide) (PLGA), a biocompatible and biodegradable polyester co-polymer of PLA and PGA, has been recognized for its ability to deliver genes. However, gene delivery by PLGA nanoparticles is limited by their negative charge and their poor transport through mucosal barriers. In this study, PLGA nanoparticles were surface modified with cationic chitosan in an effort to improve their gene delivery capability. PLGA nanoparticles were synthesized by emulsion-diffusion-evaporation technique using PVA-chitosan (PLGA1) or PVA-chitosan-PEG (PLGA2) blend as stabilizers. This method is reproducible and produces nanoparticles with hydrodynamic diameter