Paucity of HPV-Related Head and Neck Cancers (HNC) in Nigeria.
Paucity of HPV-Related Head and Neck Cancers (HNC) in Nigeria.
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DOI：
10.1371/journal.pone.0152828
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Adebamowo CA
中科院分区：
文献类型：
--
作者：
Oga EA;Schumaker LM;Alabi BS;Obaseki D;Umana A;Bassey IA;Ebughe G;Oluwole O;Akeredolu T;Adebamowo SN;Dakum P;Cullen K;Adebamowo CA
HPV相关的头颈癌（HNC）的负担在美国和其他发达国家一直在上升，但这种趋势在非洲尚未报道。本研究的目的是评估1990年至2011年期间在尼日利亚三级卫生保健机构发现的HNC癌症病例中HPV感染的患病率。我们从尼日利亚的四家教学医院中检索了1990年至2011年间诊断的149例福尔马林固定、石蜡包埋的头颈癌肿瘤标本。123块（83%）含有适当的HNC用于分析，而DNA提取成功率为60%（90/149）。PCR扩增成功率为33%（49/149），HPV线性阵列基因分型成功率为11%（17/149）。其中喉6例，颈淋巴结3例，鼻腔2例，腮腺1例，腭1例，上颌窦1例，下颌骨1例。2例为非特异性病例，无一例来自口咽部。组织学上，41%（7/17）的成功基因分型块是鳞状细胞癌（喉6例，上颌窦1例）。在我们的研究中，我们无法在任何HNC样本中检测到HPV。我们的研究结果可能表明，在尼日利亚的成年人群中，HPV相关HNC的患病率较低。我们的研究结果提供了一个基准，以比较未来的发病率HPV相关的HNC在这个社区的未来。我们面临着来自可能较差的组织处理的重大分析挑战，并敦促未来的研究应前瞻性地收集样本并确保高质量的样本处理。
The burden of HPV-related Head and Neck Cancers (HNC) has been rising in the U.S. and other developed countries but this trend has not been reported in Africa. Objective of study was to evaluate the prevalence of HPV infection in HNC cancer cases seen between 1990 and 2011 at the tertiary health care institutions in Nigeria. We retrieved 149 head and neck cancer formalin fixed, paraffin embedded tumor specimens diagnosed between 1990 and 2011 from four teaching hospitals in Nigeria. One hundred and twenty-three blocks (83%) contained appropriate HNC for analysis while DNA extraction was successful in 60% (90/149). PCR amplification was successful in 33% (49/149) and Linear Array genotyping for HPV was successful in 11% (17/149) of these cases. These were in tumors from the larynx (6), cervical lymph nodes (3), nasal cavity (2), parotid (1), palate (1), maxillary sinus (1) and mandible (1). Two cases were non-specific and none were from the oropharynx. Histologically, 41% (7/17) of the successfully genotyped blocks were squamous cell carcinomas (larynx 6, maxillary sinus 1). We were unable to detect HPV in any of the HNC samples in our study. Our result may suggest that there is a low prevalence of HPV-related HNC among the adult population in Nigeria. Our results provide a benchmark to compare future incidence of HPV -related HNC in this community in future. We had significant analytical challenges from possible poor tissue processing and urge that future studies should prospectively collect samples and ensure high quality sample processing.