Phototoxic effects of fluorescent protein KillerRed on tumor cells in mice
Phototoxic effects of fluorescent protein KillerRed on tumor cells in mice
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DOI：
10.1002/jbio.201200056
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发表时间：
2013-03-01
影响因子：
2.8
通讯作者：
Zagaynova, Elena V.
中科院分区：
文献类型：
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作者：
Shirmanova, Marina V.;Serebrovskaya, Ekaterina O.;Zagaynova, Elena V.
KillerRed 是一种独特的红色荧光蛋白，具有很强的光毒性。此前已证明其在体外杀死细菌和癌细胞的有效性。在这里，我们研究了该蛋白质对小鼠肿瘤异种移植物的光毒性。使用在线粒体中稳定表达 KillerRed 并与组蛋白 H2B 融合的 HeLa京都细胞系。用593 nm激光照射肿瘤导致KillerRed光漂白，表明光敏反应，并引起细胞的显着破坏和细胞凋亡的激活。营养不良改变的细胞比例从9.9%增加到63.7%，具有凋亡标志的细胞从6.3%增加到14%。这项研究的结果表明 KillerRed 作为一种潜在的基因编码光敏剂用于癌症光动力疗法。 （(c) 2013 WILEY-VCH Verlag GmbH & Co. KGaA，魏因海姆）
KillerRed is known to be a unique red fluorescent protein displaying strong phototoxic properties. Its effectiveness has been shown previously for killing bacterial and cancer cells in vitro. Here, we investigated the photototoxicity of the protein on tumor xenografts in mice. HeLa Kyoto cell line stably expressing KillerRed in mitochondria and in fusion with histone H2B was used. Irradiation of the tumors with 593 nm laser led to photobleaching of KillerRed indicating photosensitization reaction and caused significant destruction of the cells and activation of apoptosis. The portion of the dystrophically changed cells increased from 9.9% to 63.7%, and the cells with apoptosis hallmarks from 6.3% to 14%. The results of this study suggest KillerRed as a potential genetically encoded photosensitizer for photodynamic therapy of cancer. ((c) 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)