Precise measurement of the E-cadherin repressor Snail in formalin-fixed endometrial carcinoma using protein lysate microarrays
Precise measurement of the E-cadherin repressor Snail in formalin-fixed endometrial carcinoma using protein lysate microarrays
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DOI：
10.1007/s10585-008-9149-3
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发表时间：
2008-10-01
影响因子：
4
通讯作者：
Becker, Karl-Friedrich
中科院分区：
文献类型：
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作者：
Hipp, Susanne;Walch, Axel;Becker, Karl-Friedrich
锌指转录因子Snail的过度表达导致上皮蛋白E-钙粘蛋白和细胞角蛋白18的下调以及间充质蛋白波形蛋白的上调。我们研究的目的是首次使用蛋白质裂解物微阵列表征福尔马林固定的原发性肿瘤组织中蜗牛蛋白的表达，并将蜗牛与E-钙粘蛋白，细胞角蛋白18和波形蛋白蛋白的丰度相关联。在第一项可行性研究中，我们通过蛋白质裂解物微阵列检查了17例福尔马林固定的子宫内膜样腺癌。Snail表达与E-cadherin表达呈显著负相关（P < 0.001）。观察到Snail和细胞角蛋白18（P = 0.043）与肿瘤分级（P = 0.074）之间的相关性趋势。对于Snail和波形蛋白，未发现相关性（P = 0.384）。总之，我们的研究结果适合提出的功能蜗牛作为一个转录抑制剂的E-钙粘蛋白和细胞角蛋白18在原发性人类癌症，并证明有用的蛋白质裂解物微阵列的精确测定蛋白质参与上皮间质转化。
Over-expression of the zinc finger transcription factor Snail leads to down-regulation of the epithelial proteins E-cadherin and Cytokeratin 18 and to upregulation of the mesenchymal protein Vimentin. The aim of our study was to characterize for the first time Snail protein expression in formalin-fixed primary tumour tissues using protein lysate microarrays and correlate Snail with E-cadherin, Cytokeratin 18, and Vimentin protein abundances. In a first feasibility study, we examined 17 formalin-fixed endometrioid adenocarcinomas by protein lysate microarrays. Snail expression showed a statistical significant inverse correlation with the expression of E-cadherin (P < 0.001). A trend for correlation between Snail and Cytokeratin 18 (P = 0.043) and the tumour grade (P = 0.074) was seen. For Snail and Vimentin no correlation was found (P = 0.384). In conclusion, our results fit to the proposed function of Snail as a transcriptional repressor of E-cadherin and Cytokeratin 18 in primary human carcinomas and demonstrate the usefulness of protein lysate microarrays for the precise determination of proteins involved in epithelial-mesenchymal-transition.