Scaling single-cell genomics from phenomenology to mechanism.
Scaling single-cell genomics from phenomenology to mechanism.
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DOI：
10.1038/nature21350
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发表时间：
2017-01-18
期刊：
影响因子：
64.8
通讯作者：
Regev A
中科院分区：
文献类型：
--
作者：
Tanay A;Regev A
生物学中的三个基本问题是单个细胞如何分化形成组织，组织如何以协调和灵活的方式发挥作用，以及哪些基因调控机制支持这些过程。单细胞基因组学通过将基因组学的综合性质与描述复杂多细胞系统所需的微观分辨率相结合，为解决这些问题开辟了新的途径。早期的单细胞基因组研究为我们提供了非常丰富的异质细胞状态的现象学，但将观察研究转化为组织中的动力学和因果机制模型提出了新的挑战，需要理论，计算和实验框架之间更强的整合。
Three fundamental questions in biology are how do individual cells differentiate to form tissues, how do tissues function in a coordinated and flexible fashion, and which gene regulatory mechanisms support these processes. Single cell genomics open new ways to tackle these questions by combining the comprehensive nature of genomics with the microscopic resolution required to describe complex multi-cellular systems. Early single cell genomic studies provides us with remarkably rich phenomenology of heterogeneous cellular states, but transforming observational studies to models of dynamics and causal mechanism in tissues poses new challenges and require stronger integration between theoretical, computational and experimental frameworks.