EXPRESSION OF VARIOUS MICROTUBULE-ASSOCIATED PROTEIN-2 FORMS IN THE DEVELOPING MOUSE-BRAIN AND IN CULTURED NEURONS AND ASTROCYTES
EXPRESSION OF VARIOUS MICROTUBULE-ASSOCIATED PROTEIN-2 FORMS IN THE DEVELOPING MOUSE-BRAIN AND IN CULTURED NEURONS AND ASTROCYTES
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DOI：
10.1111/j.1471-4159.1991.tb08163.x
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发表时间：
1991-02-01
影响因子：
4.7
通讯作者：
NUNEZ, J
中科院分区：
文献类型：
--
作者：
CHARRIEREBERTRAND, C;GARNER, C;NUNEZ, J
用微管相关蛋白2(MAP2)特异的基因探针研究MAP2高、低分子突变体的mRNAs在培养神经元和星形胶质细胞中的表达。这些MAP2转录本及其编码蛋白的时间和相对丰度也在发育中的小鼠大脑半球和小脑中进行了研究。在培养的星形胶质细胞中表达了一个9kb的mRNA，已知编码高分子量的MAP2，尽管表达水平低于神经元。最近发现的编码低分子MAP2(MAP2c)的6kb转录本在神经元中表达，是星形胶质细胞主要的MAP2转录本。在星形胶质细胞和神经细胞培养的后期，9kb和6kb转录本的水平下降。在各发育阶段的小脑和大脑半球均可检测到9-kb基因的表达。虽然该基因在大脑半球的表达水平略有不同，但在小脑中的表达是双向的。这可能是由于这两个脑区形成的不同类型神经细胞的发育时间不同所致。6kb的转录本仅在这两个大脑区域的早期发育阶段被检测到。将9-kb mRNA的时间表达与高分子量MAP2的表达联系起来，表明该蛋白的积累部分是在细胞质水平上调节的。
A cDNA probe specific to microtubule-associated protein 2 (MAP2) was used to study the expression of the mRNAs encoding the high- and low-molecular-weight MAP2 variants in cultured neurons and astrocytes. The timing and relative abundance of these MAP2 transcripts and of their encoded proteins were also studied in the developing cerebral hemispheres and cerebellum of the mouse. A 9-kb mRNA, known to encode high-molecular-weight MAP2, was expressed in cultured astrocytes, albeit at a lower level than in neurons. The 6-kb transcript, recently shown to encode low-molecular-weight MAP2 (MAP2c), was expressed in neurons and was the predominant MAP2 transcript of the astrocytes. The level of the 9- and 6-kb transcripts decreased at late stages of astroglial and neuronal cell culture. The 9-kb mRNA was detected in the cerebellum and cerebral hemispheres at every developmental stage. Although the levels of this mRNA varied slightly in the cerebral hemispheres, its expression was biphasic in the cerebellum. This might be explained by the differences in timing of development of the various neuronal cell types formed in these two brain areas. The 6-kb transcript was detected only at early developmental stages in the two brain areas. Correlating the temporal expression of the 9-kb mRNA to that of high-molecular-weight MAP2 indicates that the accumulation of this protein is in part regulated at a cytoplasmic level.