A mammalian organelle map by protein correlation profiling
A mammalian organelle map by protein correlation profiling
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DOI：
10.1016/j.cell.2006.03.022
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发表时间：
2006-04-07
期刊：
影响因子：
64.5
通讯作者：
Mann, M
中科院分区：
文献类型：
--
作者：
Foster, LJ;de Hoog, CL;Mann, M
蛋白质定位于膜封闭的细胞器是细胞组织的中心特征。使用蛋白质相关性分析，我们已经将1，404种蛋白质映射到小鼠肝脏中的10个亚细胞位置，这些与酶测定，标记蛋白质谱和共聚焦显微镜相对应。这些本地化允许在已发表的细胞器蛋白质组库存的特异性评估，并证明39%的所有细胞器蛋白质的多个位置。蛋白质组学和基因组学数据的整合使我们能够识别共表达基因、顺式调控基序和参与细胞器生物发生的假定转录调控因子的网络。我们的分析将生物化学，细胞生物学和基因组学结合到细胞器分析的共同框架中。
Protein localization to membrane-enclosed organelles is a central feature of cellular organization. Using protein correlation profiling, we have mapped 1,404 proteins to ten subcellular locations in mouse liver, and these correspond with enzymatic assays, marker protein profiles, and confocal microscopy. These localizations allowed assessment of the specificity in published organellar proteomic inventories and demonstrate multiple locations for 39% of all organellar proteins. Integration of proteomic and genomic data enabled us to identify networks of coexpressed genes, cis-regulatory motifs, and putative transcriptional regulators involved in organelle biogenesis., Our analysis ties biochemistry, cell biology, and genomics into a common framework for organelle analysis.