Isolation and characterization of a novel organic solvent-tolerant Anoxybacillus sp. PGDY12, a thermophilic Gram-positive bacterium
Isolation and characterization of a novel organic solvent-tolerant Anoxybacillus sp. PGDY12, a thermophilic Gram-positive bacterium
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新型耐有机溶剂厌氧芽孢杆菌的分离和表征。
DOI：
10.1111/j.1365-2672.2010.04903.x
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发表时间：
2011-02-01
影响因子：
4
通讯作者：
Xu, T.
中科院分区：
文献类型：
--
作者：
Gao, Y.;Dai, J.;Xu, T.
目的：分离和鉴定能够在高温下耐受高浓度有机溶剂的新细菌。方法和结果：从55 ℃的温泉样品中分离到一株耐溶剂、嗜热的细菌。菌株PGDY 12被表征为革兰氏阳性细菌。它能够耐受100%的溶剂，如甲苯，苯和对二甲苯的平板覆盖和高浓度的这些溶剂在液体培养物。生长的比较表明，0.2%（v/v）的苯和0.15%（v/v）的对二甲苯能够提高最终的细胞产量。透射电子显微镜下可见细胞内电子透明物质增厚，细胞质变形。基于16 S rRNA序列数据的系统发育分析表明，菌株PGDY 12是属Anoxybacillus.Conclusions的成员：嗜热，革兰氏阳性Anoxybacillussp.PGDY12表现出独特的和显着的能力，耐受溶剂在55 ℃的意义和影响的研究：溶剂耐受性的特性是不太知道在嗜热菌。Anoxybacillussp.PGDY12是第一种能够耐受广泛溶剂的严格嗜热细菌。该菌株是一种很有前途的候选人，用作高温生物催化剂在生物技术应用。
Aims: To isolate and characterize new bacteria capable of tolerating high concentrations of organic solvents at high temperature.Methods and Results: A solvent-tolerant, thermophilic bacterium was isolated from hot spring samples at 55 degrees C. The strain PGDY12 was characterized as a Gram-positive bacterium. It was able to tolerate 100% solvents, such as toluene, benzene and p-xylene on plate overlay and high concentrations of these solvents in liquid cultures. A comparison of growth showed that 0.2% (v/v) benzene and 0.15% (v/v) p-xylene were capable of enhancing the final cell yields. Transmission electron micrographs showed the incrassation of electron-transparent intracellular material and the distorted cytoplasm in case of the cells grown in toluene. A phylogenetic analysis based on 16S rRNA sequence data indicated that the strain PGDY12 was member of the genus Anoxybacillus.Conclusions: The thermophilic, Gram-positive Anoxybacillus sp. PGDY12 exhibited a unique and remarkable ability to tolerate solvents at 55 degrees C.Significance and Impact of the Study: The solvent tolerance properties are less known in thermophilic bacteria. The Anoxybacillus sp. PGDY12 is the first strictly thermophilic bacterium able to tolerate a broad range of solvents. This strain is a promising candidate for use as a high temperature biocatalyst in the biotechnological applications.