Fluorescence correlation spectroscopy: a review of biochemical and microfluidic applications.

Fluorescence correlation spectroscopy: a review of biochemical and microfluidic applications.
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DOI:
10.1366/10-06224
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发表时间:
2011-04
影响因子:
3.5
通讯作者:
Pappas D
Pappas D
中科院分区:
化学3区
文献类型:
--
作者:
Tian Y;Martinez MM;Pappas D

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多年来,荧光相关光谱(FCS)已被证明是一种有用的技术,已被用于几个研究领域。虽然FCS最初遭受的信号噪声比差,共聚焦显微镜的结合克服了这一缺点,并将FCS转化为一个敏感的技术,具有高品质的数字。此外,串联方法已经发展到包括双色互相关,全内反射荧光相关,荧光寿命相关光谱结合时间相关单光子计数。在这篇综述中,我们讨论了几个应用FSC的生化,微流体和细胞的调查。
Over the years fluorescence correlation spectroscopy (FCS) has proven to be a useful technique that has been utilized in several fields of study. Although FCS initially suffered from poor signal to noise ratios, the incorporation of confocal microscopy has overcome this drawback and transformed FCS into a sensitive technique with high figures of merit. In addition, tandem methods have evolved to include dual-color cross-correlation, total internal reflection fluorescence correlation, and fluorescence lifetime correlation spectroscopy combined with time-correlated single photon counting. In this review, we discuss several applications of FSC for biochemical, microfluidic, and cellular investigations.
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