Transient replication of bovine papilloma virus type 1 plasmids: cis and trans requirements.
Transient replication of bovine papilloma virus type 1 plasmids: cis and trans requirements.
复制标题
牛乳头状瘤病毒 1 型质粒的瞬时复制：顺式和反式要求。
DOI：
10.1073/pnas.83.11.3609
复制
发表时间：
1986
影响因子：
11.1
通讯作者：
Botchan,MR
中科院分区：
文献类型：
--
作者：
Lusky,M;Botchan,MR
瞬时试验已被用来研究牛乳头瘤病毒1型（BPV-1）的复制。我们发现BPV-1的早期复制比细胞DNA合成更快。初始复制事件依赖于由BPV-1 E1开放阅读框编码的基因产物。病毒上游调控区的突变分析表明，复制需要两个顺式结构域。位于病毒69%转化片段外部的结构域1是增强子样活性，并且可以被其他已知的病毒增强子取代。结构域2位于先前定义为质粒维持序列1的序列内。复制对近端增强子功能的明显要求可以解释为什么某些BPV-1结构在与细菌质粒序列连接时可以在染色体外维持，而其他结构则不能。
A transient assay has been used to study bovine papilloma virus type 1 (BPV-1) replication. We show that BPV-1 early replication occurs faster than cellular DNA synthesis. Initial replication events are dependent on a gene product(s), encoded by the BPV-1 E1 open reading frame. Mutational analysis of the viral upstream regulatory region shows the requirement of two domains in cis for replication. Domain one, located outside of the viral 69% transforming fragment, is an enhancer-like activity and can be replaced by other known viral enhancers. Domain two lies within sequences previously defined as plasmid maintenance sequence 1. The apparent requirement for a proximal enhancer function for replication may explain why certain BPV-1 constructions, when linked to bacterial plasmid sequences, can be maintained extrachromosomally while others cannot.