Mice lack of LRG-47 display the attenuated outcome of infection with Schistosoma japonicum
Mice lack of LRG-47 display the attenuated outcome of infection with Schistosoma japonicum
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DOI：
10.1007/s00436-015-4853-9
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发表时间：
2016-03
影响因子：
2
通讯作者：
Yanan Gao;Jingjiao Wu;Meijuan Zhang;M. Hou;M. Ji
中科院分区：
文献类型：
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作者：
Yanan Gao;Jingjiao Wu;Meijuan Zhang;M. Hou;M. Ji
干扰素诱导型GTdR LRG-47（也称为免疫相关GTdR M，Irgm 1）是p47 GTdR家族的一员，已被证明可调节宿主对细胞内病原体的抗性。关于LRG-47在宿主对细胞外病原体应答中的作用知之甚少。为了研究LRG-47在日本血吸虫感染过程中的可能作用，用日本血吸虫尾蚴攻击LRG-47缺陷型（LRG-47-/-）和野生型（WT）小鼠。日本血吸虫感染小鼠后，对小鼠的细胞和体液免疫应答进行了分析。在S.与WT小鼠相比，LRG-47−/−小鼠表现出显著降低的卵负荷、低的溶酶体特异性抗体应答以及降低的Th 1和增加的Tc 1应答。此外，日本血吸虫虫卵抗原免疫也产生了与S类似的体液和细胞免疫应答。日本血吸虫感染综上所述，LRG-47的缺陷可能通过减弱IFN-γ下游信号传导而影响宿主的CD 4 +T细胞免疫应答，但LRG-47在处理细胞外蠕虫方面的特异性功能有待进一步研究。
Interferon-inducible GTPase LRG-47 (also named immune-related GTPase M, Irgm1) is a member of the p47 GTPase family that has been shown to regulate host resistance to intracellular pathogens. Little knowledge has been known about the role of LRG-47 in host’s responses to extracellular pathogens. To investigate possible roles of LRG-47 in the course ofSchistosoma japonicuminfection, LRG-47-deficient (LRG-47−/−) and wild-type (WT) mice were challenged with cercariae ofS. japonicum, and the cellular and humoral responses in mice were analyzed. At the acute stage ofS. japonicuminfection, in contrast to WT mice, LRG-47−/−mice showed the significantly decreased egg burden, low schistosome-specific antibody response, and the decreased Th1 and increased Tc1 responses. Additionally,Schistosoma japonicum-specific egg antigen immunization also produced the similar humoral and cellular immune responses asS. japonicuminfection. Taken together, these data suggested that the deficiency of LRG-47 might affect host’s CD4+T cell immune response via the weakening of IFN-γ downstream signaling; however, the specific function of LRG-47 on dealing with extracellular worm needs to be further studied.