BEN1, a gene encoding a dihydroflavonol 4-reductase (DFR)-like protein, regulates the levels of brassinosteroids in Arabidopsis thaliana.
BEN1, a gene encoding a dihydroflavonol 4-reductase (DFR)-like protein, regulates the levels of brassinosteroids in Arabidopsis thaliana.
复制标题
DOI：
10.1111/j.1365-313x.2007.03129.x
复制
发表时间：
2007-07
期刊：
影响因子：
--
通讯作者：
T. Yuan;S. Fujioka;S. Takatsuto;S. Matsumoto;X. Gou;Kai He;S. Russell;Jia Li
中科院分区：
文献类型：
--
作者：
T. Yuan;S. Fujioka;S. Takatsuto;S. Matsumoto;X. Gou;Kai He;S. Russell;Jia Li
ben 1 -1D（bri 1 -5增强1- 1显性）突变体通过bri 1 -5基因外修饰物的激活标记筛选而被鉴定。bri 1 -5是油菜素类固醇受体基因BRI 1的弱突变等位基因。BEN 1的过表达大大增强了bri 1 -5植物的缺陷表型。另一方面，在Col-0野生型背景中通过基因破坏去除BEN 1促进器官的伸长。由于BEN 1编码一种与二氢黄酮醇4-还原酶（DFR）和花青素还原酶（BAN）同源的新蛋白，BEN 1可能参与油菜素类固醇代谢途径。对油菜素类固醇概况的分析表明，BEN 1确实负责调节几种油菜素类固醇的水平，包括typhasterol，castasterone和油菜素内酯。在体内饲养和体外生化分析表明，BEN 1可能参与了一个新的机制，以调节油菜素类固醇水平。这些结果提供了额外的洞察生物活性油菜素类固醇的调节机制。
The ben1-1D (bri1-5 enhanced 1-1dominant) mutant was identified via an activation-tagging screen for bri1-5 extragenic modifiers. bri1-5 is a weak mutant allele of the brassinosteroid receptor gene, BRI1. Overexpression of BEN1 greatly enhances the defective phenotypes of bri1-5 plants. Removal of BEN1 by gene disruption in a Col-0 wild-type background, on the other hand, promotes the elongation of organs. Because BEN1 encodes a novel protein homologous to dihydroflavonol 4-reductase (DFR) and anthocyanidin reductase (BAN), BEN1 is probably involved in a brassinosteroid metabolic pathway. Analyses of brassinosteroid profiles demonstrated that BEN1 is indeed responsible for regulating the levels of several brassinosteroids, including typhasterol, castasterone and brassinolide. In vivo feeding and in vitro biochemical assays suggest that BEN1 is probably involved in a new mechanism to regulate brassinosteroid levels. These results provide additional insight into the regulatory mechanisms of bioactive brassinosteroids.