Gluconeogenesis from glycerol at rest and during exercise in normal, diabetic, and methylprednisolone-treated dogs.
Gluconeogenesis from glycerol at rest and during exercise in normal, diabetic, and methylprednisolone-treated dogs.
复制标题
正常、糖尿病和甲基强的松龙治疗的狗在休息和运动期间甘油的糖异生作用。
DOI：
10.1016/0026-0495(76)90091-3
复制
发表时间：
1976
期刊：
影响因子：
--
通讯作者：
B. Issekutz
中科院分区：
文献类型：
--
作者：
W. A. Shaw;T. Issekutz;B. Issekutz
在正常(N)、四氧嘧啶糖尿病(D)和甲基强的松龙治疗的糖尿病（MPD）犬的休息和运动（跑步机）期间，采用示踪技术（启动恒速率输注），使用2- 3h葡萄糖和14c -甘油，测量葡萄糖周转率、甘油周转率和甘油碳掺入葡萄糖的速率。在休息时，只有2%-3%的肝脏葡萄糖输出来自甘油。运动使N组的狗的糖异生增加了约9倍，D组和MPD组的狗的糖异生增加了约4倍，但葡萄糖转化的增加中不到9%是由甘油引起的。在所有三组中，甘油周转率和甘油在休息和运动时的糖异生之间存在直接的线性相关。然而，N、D和MPD犬的斜率常数差异显著：分别为0.45、0.51和0.67。在体内，控制甘油糖异生率的主要因素似乎是甘油的供应，胰岛素缺乏和糖皮质激素治疗的特殊作用叠加在甘油供应上。它们似乎不太重要。通过比较2- 3h -葡萄糖法和6- 3h -葡萄糖法测定的葡萄糖周转，发现D犬的葡萄糖-葡萄糖-6- p循环活性高3倍，MPD动物的葡萄糖-葡萄糖-6- p循环活性高15倍。
Glucose turnover, glycerol turnover, and the rate of incorporation of glycerol carbon into glucose were measured with the tracer technique (primed constant rate infusion) using 2-3H-glucose and14C-glycerol, at rest and during exercise (treadmill run) in normal (N), alloxan-diabetic (D), and methylprednisolone treated diabetic (MPD) dogs. At rest only 2%–3% of the hepatic glucose output arose from glycerol. Exercise increased gluconeogenesis about ninefold in N dogs and about fourfold in D and MPD animals, yet <9% of the elevated glucose turnover was derived from glycerol. There was a direct linear correlation between the rates of glycerol turnover and gluconeogenesis from glycerol at rest and during exercise in all three groups. The slope constants were however significantly different: 0.45, 0.51, and 0.67 for N, D, and MPD dogs, respectively. In vivo the major factor controlling the rate of gluconeogenesis from glycerol seems to be the glycerol supply on which the specific effects of insulin deficiency and glucocorticoid treatment are superimposed. They appear to be of minor importance. A comparison of the glucose turnover measured by 2-3H-glucose with that measured by 6-3H-glucose showed that the activity of the glucose glucose-6-P cycle was threefold higher in D dogs and elevated by 15-fold in MPD animals.