SELECTION INVITRO OF SINGLE-STRANDED-DNA MOLECULES THAT FOLD INTO SPECIFIC LIGAND-BINDING STRUCTURES
SELECTION INVITRO OF SINGLE-STRANDED-DNA MOLECULES THAT FOLD INTO SPECIFIC LIGAND-BINDING STRUCTURES
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DOI：
10.1038/355850a0
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发表时间：
1992-02-27
期刊：
影响因子：
64.8
通讯作者：
SZOSTAK, JW
中科院分区：
文献类型：
--
作者：
ELLINGTON, AD;SZOSTAK, JW
我们已经通过体外选择和扩增从大量随机序列DNA中分离出一组配体结合DNA序列，使用与分离配体结合RNA 1所述的方法相似的方法。配体-DNA相互作用是序列特异性和配体特异性的，并且依赖于单链DNA的正确折叠。一些配体导致分离出比RNA序列更多的DNA序列，反之亦然。单个序列的分析表明，配体结合是DNA特异性的;相同序列的RNA不能与相同的配体相互作用。配体结合DNA可能比RNA更适合作为潜在的药理学试剂2-4，因为DNA的稳定性更高。RNA在生命早期进化中的明显主导地位5-7可能是由于其可用性，而不是其功能优越性。
WE have isolated a set of ligand-binding DNA sequences from a large pool of random sequence DNAs by selection and amplification in vitro, using similar methods to those described for the isolation of ligand-binding RNAs 1. The ligand-DNA interactions are both sequence- and ligand-specific, and are dependent on proper folding of the single-stranded DNA. Some ligands led to the isolation of more DNA sequences than RNA sequences, and vice versa. Analysis of individual sequences reveals that ligand binding is DNA-specific; RNAs of identical sequence could not interact with the same ligands. Ligand-binding DNAs might be more suitable than RNAs as potential pharmacological reagents 2-4 because of the greater stability of DNA. The apparent primacy of RNA in the early evolution of life 5-7 may have been due to its availability rather than to its functional superiority.